Naslov
Activation of αSMA expressing perivascular cells during reactionary dentinogenesis

Autori
Vidovic-Zdrilic, I. ; Vijaykumar, A. ; Mina, M.

Izvornik
International Endodontic Journal (0143-2885) 52 (2019), 1; 68-76

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
odontoblasts ; perivascular cells ; pulp biology ; reactionary dentine ; αSMA

Sažetak
Aim: To examine the contribution of perivascular cells expressing αSMA to reactionary dentinogenesis. Methodology: An inducible, Cre- loxP in vivo fate- mapping approach was used to examine the contribution of the descendants of cells expressing the αSMA-CreERT2 transgene to reactionary dentinogenesis in mice molars. Reactionary dentinogenesis was induced by experimental mild injury to dentine without pulp exposure. The Student's t test was used to determine statistical significance at *P ≤ 0.05. Results: The lineage tracing experiments revealed that mild injury to dentine first led to activation of αSMA-tdTomato+ cells in the entire pulp chamber. The percentage of areas occupied by αSMA-tdTomato+ in injured (7.5 ± 0.7%) teeth were significantly higher than in teeth without injury (2 ± 0.5%). After their activation, αSMA- tdTomato+ cells migrated towards the site of injury, gave rise to pulp cells and a few odontoblasts that became integrated into the existing odontoblast layer expressing Col2.3-GFP and Dspp. Conclusion: Mild insult to dentine activated perivascular αSMA-tdTomato+ cells giving rise to pulp cells as well as a few odontoblasts that were integrated into the pre-existing odontoblast layer.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA