Naslov
Curcumin - A Potent Inhibitor of Galectin-3 Expression

Autori
Dumić, Jerka ; Dabelić, Sanja ; Flögel, Mirna

Izvornik
Food technology and biotechnology (1330-9862) 40 (2002), 4; 281-287

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
galectin-3; curcumin; alkylatingagent MNNG; UV light

Sažetak
The expression of galectin-3, a P-galactoside binding lectin, was found to be affected by different kinds of stressors, and is strongly modified in numerous physiological and pathophysiological conditions. Although no precise regulatory mechanisms of galectin-3 expression are unraveled, transcription factors AP-1 (activator protein 1) and NF-kappaB (nuclear factor kappa B) play an important role. in these processes. Activities of both transcription factors are affected by curcumin, a biologically active compound extracted from rhizomes of Curcuma species. We have analyzed the impact of curcumin on the expression of galectin-3 in glioblastoma cells under basal conditions and under stress invoked by the cell exposure to alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and ultraviolet C (UV-C) light. Galectin-3 level was measured by western-blot technique using M3/38 monoclonal antibody. Curcumin has decreased the basal level of galectin-3, while the pretreatment of cells with curcumin has considerably reduced the inducible effect of UV-C radiation and abolished the inducible effect of alkylating agent. Thus, curcumin has been identified as a potent inhibitor of galectin-3 expression.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA