Pregled bibliografske jedinice broj: 1046426
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia
The chemical species of mercury accumulated by Pseudomonas idrijaensis, a bacterium from a rock of the Idrija mercury mine, Slovenia // Chemosphere, 248 (2020), 126002, 10 doi:10.1016/j.chemosphere.2020.126002 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1046426 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The chemical species of mercury accumulated by
Pseudomonas idrijaensis, a bacterium from a rock
of the Idrija mercury mine, Slovenia
Autori
Bourdineaud, Jean-Paul ; Durn, Goran ; Režun, Bojan ; Manceau, Alain ; Hrenović, Jasna
Izvornik
Chemosphere (0045-6535) 248
(2020);
126002, 10
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Bacteria ; Pseudomonas idrijaensis ; resistance ; mercury ; rock
Sažetak
A mercury-resistant bacterial strain has been isolated from a rock of the Idrija mercury mine in Slovenia. The rock had 19 g carbon and 2952 mg mercury (Hg) per kg. Mass spectrometry and DNA sequencing showed that the bacterium belongs to the Pseudomonas genus. It is called Pseudomonas idrijaensis. This bacterial strain is sensitive to methylmercury (MeHg) like the reference P. aeruginosa strain PAO1, and is resistant to divalent mercury (Hg(II)) in contrast to PAO1. This difference could be attributed to the presence of the mer operon yet deprived of the merB gene encoding the organomercurial lyase, on the basis of whole genome sequencing. The P. idrijaensis mer operon displays the RTPCADE organization and is contained in the Tn5041 transposon. This transposon identified here occurs in other Gram- negative Hg-resistant strains isolated from mercury ores, aquatic systems and soils, including Pseudomonas strains from 15, 000 to 40, 000 years old Siberian permafrost. When P. idrijaensis was exposed to mercury chloride, two intracellular Hg species were identified by high energy-resolution XANES spectroscopy, a dithiolate Hg(SR)2 and a tetrathiolate Hg(SR)4 complex. P. idrijaensis had a much higher [Hg(SR)2]/[Hg(SR)4] molar ratio than bacteria lacking the mer operon when exposed to 4 μg Hg2+/L - resulting in an intracellular accumulation of 4.3 μg Hg/g dw. A higher amount of the Hg(SR)2 complex provides a chemical signature for the expression of the dicysteinate Mer proteins in response to mercury toxicity.
Izvorni jezik
Engleski
Znanstvena područja
Geologija, Biologija
POVEZANOST RADA
Projekti:
HRZZ-IP-2014-09-5656 - Prirodno stanište klinički značajnih Acinetobacter baumannii (NATURACI) (Hrenović, Jasna, HRZZ - 2014-09) ( CroRIS)
Ustanove:
Prirodoslovno-matematički fakultet, Zagreb,
Rudarsko-geološko-naftni fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
