Naslov
Influence of pioglitazone on the microglial reaction and some markers of inflammation and cell stress in the rat hippocampus following lateral fluid percussion injury

Autori
Slavić, Ante ; Delač, Ljerka ; Dolenec, Petra ; Župan, Gordana ; Pilipović, Kristina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9. Hrvatski kongres farmakologije s međunarodnim sudjelovanjem - zbornik sažetaka / - Zagreb : Hrvatsko društvo farmakologa, 2019, 151-151

Skup
9. hrvatski kongres farmakologije = 9th Croatian Congress of Pharmacology

Mjesto i datum
Zagreb, Hrvatska, 25.09.2019. - 28.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hippocampus ; microglia ; pioglitazone ; traumatic brain injury

Sažetak
Introduction: One of the leading causes of death and disability throughout the world, traumatic brain injury (TBI), still lacks clinically effective neuroprotective therapy. Previously it was found that, in the TBI in rats, application of a single dose of pioglitazone, a peroxisome proliferator- activated receptor-γ (PPARγ) agonist, had some limited beneficial effects on trauma-induced oxidative hippocampal damage and neurodegeneration. The aim of this study was to examine the effects of pioglitazone, applied twice daily, on the microglial activation and the expressions of inflammation (COX-2) and cell stress (HSP70) markers in the rat hippocampus following lateral fluid percussion injury (LFPI). Materials and methods: Moderate brain trauma was performed over the left parietal cortex by the LFPI method in adult male Wistar rats. Pioglitazone or vehicle were administered i.p. 10 min and 12 h after the injury. Sham- operated, vehicle treated animals were used as the control group. Rats were sacrificed 24 h after TBI and their hippocampi were prepared for the histological and western blotting analyses. Results: Pioglitazone significantly decreased TBI-induced microglial activation in the dentate gyrus of the hippocampus. TBI caused an increase in the expressions of COX-2 and HSP70, but the applied PPARγ agonist did not have an effect on the levels of these proteins. Conclusions: Our study showed that pioglitazone, applied twice daily, reduced microglial reaction in the hippocampus after TBI in the rat, but did not influence the levels of measured inflammatory and cell stress markers. This work was supported by the University of Rijeka, Croatia, project number uniri-biomed- 18-204 to Ž.G.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA