Pregled bibliografske jedinice broj: 1040822
Environmental DNA in subterranean biology update: from “Where?” to “How many?”
Environmental DNA in subterranean biology update: from “Where?” to “How many?” // ARPHA Conference Abstracts (ACA)
Aveiro, Portugal, 2018. e29968, 1 doi:10.3897/aca.1.e29968 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1040822 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Environmental DNA in subterranean biology update: from “Where?” to “How many?”
Autori
Gorički, Špela ; Presetnik, Primož ; Prosenc- Zmrzljak, Uršula ; Blatnik, Matej ; Gredar, Tajda ; Kogovšek, Blaž ; Koit, Oliver ; Strah, Sara ; Bilandžija, Helena ; Jalžić, Branko ; Aljančič, Gregor ; Košir, Rok ; Mayaud, Cyril ; Štebih, Dejan ; Hudoklin, Andrej ; Jeffery, William
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
ARPHA Conference Abstracts (ACA)
/ - , 2018
Skup
24th International Conference on Subterranean Biology
Mjesto i datum
Aveiro, Portugal, 20.08.2018. - 24.08.2018
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
cave species ; threatened ; conservation ; Dinaric karst ; distrubution range
Sažetak
Recent records of Proteus anguinus outside its historically known range (Gorički et al. 2017), discovered through detection of its DNA dissolved in groundwater (environmental DNA or eDNA), mark the beginning of a new era in the study and conservation of cryptic subterranean biodiversity. An upgraded technology, droplet digital PCR (ddPCR), initially developed for studies of gene expression, detection of genetically modified organisms and in medical diagnostics, is being tested for improved detection of the much smaller and rare stygobiont, the cave clam Congeria jalzici. In parallel to eDNA assay development for various stygobiotic species of the Dinaric Karst, a groundwater-sample library is being created. The samples will be available for future analysis of their species composition and will also serve as a source of information on any changes in species distribution over time. In another line of eDNA research, the utility of ddPCR for direct quantification of eDNA molecules in groundwater is being explored by using the large, accessible and well- characterized (Zakšek and Trontelj 2017) natural Proteus population in the Planina Cave (Slovenia) as a model. The eDNA methodology may in the future be applied in estimation and monitoring of Proteus population sizes without having to see, mark or otherwise disturb the animals themselves.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Ustanove:
Institut "Ruđer Bošković", Zagreb