Naslov
Red Walnut: Characterization of the Phenolic Profiles, Activities and Gene Expression of Selected Enzymes Related to the Phenylpropanoid Pathway in Pellicle during Walnut Development

Autori
Martina Persic ; Maja Mikulic-Petkovsek ; Heidi Halbwirth ; Anita Solar ; Robert Veberic ; Ana Slatnar

Izvornik
Journal of agricultural and food chemistry (0021-8561) 66 (2018), 11; 2742-2748

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
enzymatic activity ; walnut ; anthocyanins

Sažetak
A rare walnut variant with a red seed coat (pellicle) was examined for alterations in its phenolic profile during development. The red- walnut (RW) pellicle was compared with two commonly colored walnut varieties: ‘Lara’ (brown) and ‘Fernor’ (light brown). Furthermore, the activities of selected enzymes of the phenylpropanoid- and flavonoid-related pathways and the relative expressions of the structural genes phenylalanine ammonia lyase (PAL) and anthocyanidin synthase (ANS) were examined in the pellicles of the three varieties. In the pellicles of the RWs, phenylalanine ammonia lyase (PAL) activity and related PAL expression was most pronounced in August, about one month before commercial maturity, suggesting a high synthesis rate of phenolic compounds at this development stage. The most pronounced differences between the red and light- and dark-brown varieties were the increased PAL activity, PAL expression, and ANS expression in RWs in August. The vibrant color of the RW pellicle is based on the presence of four derivatives of cyanidin- and delphinidin- hexosides.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija, Interdisciplinarne biotehničke znanosti

POVEZANOST RADA