Naslov
Recombinogenicity of palindromes and quasipalindromes in DNA

Autori
Svetec Miklenić, M. ; Žunar, B. ; Štafa, A. ; Svetec, I.K

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
HDBMB2019 – Crossroads in life sciences / - , 2019, 45-45

Skup
HDBMB2019 – Crossroads in life sciences

Mjesto i datum
Lovran, Hrvatska, 25.09.2019. - 28.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
25. – 28. 09. 2019
(palindromes, quasipalindromes, yeast)

Sažetak
A perfect palindrome in DNA consists of two adjacent inverted repeats, while a quasipalindrome can contain a spacer region and/or mismatches between the repeats. Because of their symmetry palindromic sequences can undergo intrastrand base pairing and hence form secondary structures – hairpins in ssDNA and cruciforms in dsDNA. These structures represent physical obstacles for processes such as DNA replication and must be eliminated which can lead to a double strand break in DNA. Therefore, palindromic sequences are considered to be fragile sites in the genome, i.e. sites that are prone to chromosome breakage and subsequent genetic recombination. For example, two recombinogenic palindromes located on chromosomes 11 and 22 are the cause of the most common recurrent reciprocal translocation in the human genome which can lead to the birth of children with the Emanuel syndrome characterised by physical malformations and mental retardation. In our research we investigate various aspects of palindrome recombinogenicity using yeast Saccharomyces cerevisiae as a eukaryotic model organism. We constructed an experimental system which allows us to compare the recombinogenicity of various perfect palindromes and quasipalindromes in vivo, both by measuring the recombination rate and by simply monitoring the yeast colony colour. Using this methodology, we determined the influence of palindrome length, spacer length, growth temperature and the absence of various proteins involved in DNA repair and replication on palindrome recombinogenicity.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA