Naslov
COMPLEXITY AND DIVERSITY OF HLADRB1*04~DQB1 HAPLOTYPES IN THE CROATIAN POPULATION

Autori
Sviličić, Danijela ; Maskalan, Marija ; Burek Kamenarić, Marija ; Grubić, Zorana ; Žunec, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts for the 33rd European Immunogenetics and Histocompatibility Conference, Lisbon, Portugal, May 8‐11, 2019 / Marsh, Steven GE - , 2019, 342-342

Skup
33rd European Immunogenetics and Histocompatibility Conference

Mjesto i datum
Lisabon, Portugal, 08.05.2019. - 11.05.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HLA-DRB1*04, haplotypes, Croatian population

Sažetak
Information on the precise content of HLA haplotypes is needed for transplantation, disease association, anthropological and epidemiological studies. In this study we aimed to analyse HLA- DRB1*04~DQB1 haplotype diversity at a high- resolution level in DRB1*04-positive individuals (N=734). The high-resolution level typing of HLA- DRB1 and DQB1 loci was performed using the Polymerase Chain Reaction - Sequence Specific Primers (PCR-SSP) method. A total of 10 different DRB1*04 alleles (DRB1*04:01- 08, DRB1*04:15 and DRB1*04:50) and nine different DQB1 alleles (DQB1*03:01-05, DQB1*03:07, DQB1*02:02, *04:01-02) have been determined, composing 29 different haplotypes. The most common DRB1*04 alleles were DRB1*04:01 (30.6%), DRB1*04.02 (25.2%), DRB1*04:04 (19.7%) and DRB1*04:03 (13.5%). The most prevalent DQB1 allele was DQB1*03:02 (85%), followed by DQB1*03:01 (7.5%). The two most frequent haplotypes were DRB1*04:01~DQB1*03:02 and DRB1*04:02~ DQB1*03:02 (35.2% and 27.4%, respectively). The haplotype analysis performed for each respective HLA-DRB1*04 allele revealed the most prevalent association with DQB1*03:02 for most of them. The most striking one was exclusive association of DRB1*04:02 with DQB1*03:02, as it was observed in all DRB1*04:02 positive cases (N=201). On the other side, DRB1*04:07 was most commonly found with DQB1*03:01 (83% of DRB1*04:07 positive cases) while DRB1*04:08 was most commonly found with DQB1*03:04 (82% of DRB1*04:08 positive cases). The analysis also showed DRB1*04:03~DQB1*03:05 as an exclusive haplotype association, as it was found on all DQB1*03:05 positive haplotypes (N=9). DRB1*04:05 was the most frequent allele found in an unusual association with DQB1*02:02 (85 % of DQB1*02:02 positive haplotypes). These results provide information on HLA-DRB1*04~ DQB1 haplotype diversity in the Croatian population that can be implemented in the strategic planning in unrelated hematopoietic stem cell transplantation increasing the likelihood of finding a suitable donor.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti

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