Naslov
Quantitative PCR Technology in Chimerism Monitoring After Hsct ‐ One Centre Experience

Autori
Štingl Janković, Katarina ; Maskalan, Marija ; Burek Kamenarić, Marija ; Žunec, Renata ; Duraković, Nadira ; Serventi‐Seiwerth, Ranka ; Vrhovac, Radovan ; Grubić, Zorana

Izvornik
HLA (2059-2302) 94 (2019), S2; 16-20

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
HSCT ; chimerism ; qPCR

Sažetak
Chimerism status evaluation is a routine test performed in post-HSCT period. Aim of the study was to evaluate a qPCR method (GenDx, Utrecht, the Netherlands) applicability for this purpose. Study included 74 recipient/donor pairs tested for informative markers: median of four and six informative markers was found for patients (related and unrelated donor, respectively). Higher sensitivity of qPCR method was confirmed by analysis of recipient post-HSCT samples (N = 800) among which microchimerism (0.1-1% recipient DNA) was detected in 21.8% of cases. The ability to detect less than 1% of minor population, as opposed to the Short Tandem Repeat (STR) method for which 1% is the limit, translated into earlier identification of a disease relapse for four patients in our study sample.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti

POVEZANOST RADA