Naslov
In vitro antioxidant and antihypertensive compounds from camu-camu (Myrciaria dubia McVaugh, Myrtaceae) seed coat: A multivariate structure-activity study

Autori
Marina Fidelis, Janio Sousa Santos, Graziela Bragueto Escher, Mariana Vieira do Carmo, Luciana Azevedo, Marcia Cristina da Silva, Predrag Putnik, Daniel Granato

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
First International Congress on Cocoa Coffee and Tea Asia 2018 / - Hefei : State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University, China, 2018, 128-129

Skup
First International Congress on Cocoa Coffee and Tea Asia

Mjesto i datum
Hefei, Kina, 17.10.2018. - 20.10.2018

Vrsta sudjelovanja
Plenarno

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
In vitro ; antioxidant ; antihypertensive compounds ; camu-camu (Myrciaria dubia McVaugh Myrtaceae) ; seed coat ; multivariate structure-activity study ;

Sažetak
Camu-camu (Myrciaria dubia) pulp, seeds, and skin are widely known because of their nutritional properties. However, the seed coat has never been studied as a source of bioactive compounds. Herein, we characterized the phenolic composition, the antioxidant activity, and inhibition of angiotensin-converting enzyme (ACE) of three different extracts (water, propanone, and ethanol) from this residue and assessed the structure-activity using bivariate and multivariate statistical approaches. Phenolic acids and flavonoids were quantified by high-performance liquid chromatography while the ferric reducing antioxidant power (FRAP), inhibition of lipid peroxidation using egg yolk and Wistar rat brain, scavenging of 2, 2-diphenyl-1-picrylhydrazyl (DPPH') radical, Folin-Ciocalteu reducing capacity (FCRC), and the inhibition of angiotensin-converting enzyme (ACE) were also analyzed. t-Resveratrol was found in camu-camu seed coat for the first time. The aqueous extract had the highest total phenolic content, FRAP, DPPH·, FCRC, and inhibition of lipid oxidation using both chemical and biological assays, while the propanone extract showed the opposite behavior but it presented higher in vitro antihypertensive activity. The ethanolic extract exhibited intermediate values for the responses. Gallic acid, 2, 5-dihydroxybenzoic acid, 2, 4-dihydroxybenzoic acid, chlorogenic acid, and (-)-epicatechin were positively and significantly correlated (p<0.05) with FRAP, DPPH', and FCRC. t-Resveratrol was only significantly associated with Cu2+ chelating ability. The in vitro inhibition of ACE activity showed a significant (p<0.05) and positive correlation with the content of p-coumaric, rosmarinic, ferulic, caffeic, ellagic acids, quercetin-3-rutinoside, and quercetin. Another important result obtained here is the significant (p<0.05) correlation between the inhibition of egg yolk lipid peroxidation with FRAP (r=0.9261), DPPH (r=0.9667), and FCRC (r=0.9727). Similarly, significant (p<0.05) correlation between the inhibition of rat brain lipid oxidation was obtained with FRAP (r=0.9119), DPPH (r=0.9624), and FCRC (r=0.9429). In addition both antioxidant activity methods based on the measurement of TBARS in triacylglycerol-rich matrices (egg yolk and rat brain homogenate) were also significantly correlated (r=0.9366, p<0.001). In conclusion, the association between chemical composition and the functional properties of the camu-camu seed coat extracts were revealed using correlation analysis and principal component analysis.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

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