Naslov
Optimization of Ultrasound-Assisted Extraction of Phenolic Antioxidants from Lotus corniculatus

Autori
Fumić, Barbara ; Jug, Mario ; Zovko Končić, Marijana

Izvornik
Croatica Chemica Acta (0011-1643) 92 (2019), 3; 1-9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
genistein ; kaempferol ; Lotus corniculatus ; quercetin ; radical scavenging activity ; response surface methodology

Sažetak
Ultrasound-assisted extraction of phenolic antioxidants from Lotus corniculatus was optimized using response surface methodology. The extraction was performed according to the Box–Behnken design with ethanol concentration, temperature, and pH, as independent variables. The responses were extraction yield, DPPH radical scavenging activity (RSA) IC50 and content of different phenolic compounds (total phenols, flavonoids and phenolic acids, as well as quercetin, kaempferol and genistein derivatives). The models were used to calculate best conditions for maximal extraction of phenolic compounds and antiradical activity. Use of the optimized extraction parameters increased the content of quercetin and kaempferol derivatives more than tenfold (from 6.07 to 65.10 mg mL–1 and 6.69 to 92.75 mg mL–1, respectively). The results of this work stress the importance of careful selection of conditions for flavonoids extraction. Abundance of bioactive phenolics in L. corniculatus extracts obtained under optimized extraction conditions opens the possibility for wider utilization of this plant.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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