Naslov
Role of ganglioside GM1 expression in T lymphocytes membranes in cervical cancer development

Autori
Danolić, Damir ; Heffer, Marija ; Wagner, Jasenka ; Škrlec, Ivana ; Alvir, Ilija ; Mamić, Ivica ; Šušnjar, Lucija ; Marcelić, Luka ; Bečejac, Tomislav ; Puljiz, Mario

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
International Journal of Gynecologic Cancer / Ramirez, Pedro T - Hoboken (NJ) : BMJ, 2019

Skup
21st European Gynaecological Oncology Congress of the European Society of Gynaecological Oncology

Mjesto i datum
Atena, Grčka, 02.11.2019. - 05.11.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Cervical cancer ; ganglioside GM1 ; flow cytometry

Sažetak
Introduction/Background Cervical cancer is worldwide the second most common cancer in women. Immune response appears to be a key determinant in controlling human papilloma virus (HPV) infection. Cell mediated immunity plays a significiant role in progression or regression of neoplastic cervical lesions. Lipid rafts have essential role in T lymphocite activation. Gangliosides play an important role in the formation and stabilization of specific cell lipid membrane domains. This study aims to determine whether ganglioside GM1 expressions in T cell membrane represent risk factor for cervical cancer. Methodology In total, 20 women participated in our study. Peripheral blood samples were obtained from 10 cervical cancer patients and 10 healthy controls at University Hospital for Tumors, Zagreb, Croatia. A 3 mL sample of venous blood was collected from each subject into a test tube containing EDTA as anticoagulant. For T cell subtypes isolation we used Anti CD4 Phycoerythrin antibody and Anti CD8 Peridinin Chlorophyll Protein Complex antibody. Ganglioside GM1 expression in T lymphocytes membranes was analyzed using double labeling antibodies on ganglioside GM1. Results We have not found difference in ganglioside GM1 expression in T lymphocytes membranes between the two groups. Conclusion The present study did not show association of ganglioside GM1 expression in T lymphocytes membranes with cervical cancer development.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA