Naslov
EFFECTS OF CAFFEINE ON DEVELOPMENT OF OXIDATIVE STRESS AND CRYSTALLIZATION ON IN VITRO MODEL OF OXALATE UROLITHIASIS

Autori
Kizivat, Tomislav ; Ninčević, Vjera ; Cindrić, Anita ; Omanović Kolarić, Tea ; Kuna, Lucija ; Smolić, Robert ; Bilić-Ćurčić, Ines ; Smolić, Martina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9. Hrvatski kongres farmakologije s međunarodnim sudjelovanjem / - Zagreb, 2019, 132-132

Skup
9. hrvatski kongres farmakologije = 9th Croatian Congress of Pharmacology

Mjesto i datum
Zagreb, Hrvatska, 25.09.2019. - 28.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
caffeine ; oxidative stress ; urolithiasis

Sažetak
Introduction: Urolithiasis is a common disease characterized by formation of solid deposits in the urinary tract. Most of the stones generated inside urinary tract are predominantly composed of calcium oxalate which can produced free radicals. Aim: To study the effects of caffeine on formation of crystals and it’s antioxidative effects in an in vitro model of urolithiasis. Materials and methods: LLC-PK1 cells were used as in vitro model of urolithiasis. Oxidative stress was induced by exposing cells to crystals of calcium oxalate. Cells were treated with different concentrations of caffeine. Effects of caffeine on crystallization and oxidative stress was estimated by crystals counts and total glutathione concentrations, respectively. Results: Increasing the concentration proportionally, caffeine stops the crystallization of calcium oxalate in in vitro model of proximal kidney tubules urolithiasis. Number of calcium oxalate crystals was decreasing in correlation with increasing concentrations of caffeine up to 60% and with the length of treatment with caffeine. Concentrations of total glutathione change up to 1, 8% when caffeine is added to cells treated with calcium oxalate crystals suggesting amelioration of oxidative stress. Conclusion: Results suggest that caffeine can lower crystallization and has a positive effect by lowering oxidative stress in our in vitro models of urolithiasis. More studies are needed to further evaluate caffeine’s role in formation of kidney stones.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA