Naslov
Application of deep eutectic solvents in extraction of bioactive compounds from food industry byproduct-cocoa bean shell

Autori
Pavlović, Nika ; Jokić, Stela ; Jakovljević, Martina ; Jerković, Igor ; Molnar, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka s 12. međunarodnog znanstveno- stručnog skupa HRANOM DO ZDRAVLJA / Babić, Jurislav ; Šubarić, Drago ; Jašić Midhat - Osijek : Tuzla : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku ; Tehnološki fakultet Univerziteta u Tuzli, 2019, 166-166

Skup
12th International Scientific and Professional Conference WITH FOOD TO HEALTH

Mjesto i datum
Osijek, Hrvatska ; Tuzla, Bosna i Hercegovina, 24.10.2019. - 25.10.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cocoa bean shell, waste, deep eutectic solvents, microwave assisted extraction, active compounds

Sažetak
This is the first report on the extraction of cocoa bean shell (CBS) using deep eutectic solvents (DESs) followed by microwave extraction. CBS was extracted using 16 different choline chloride based DESs at temperature of 50 °C during 60 minutes, with 10% and 50% water addition. Identification and quantification of bioactive compounds in obtained extracts was performed by high performance liquid chromatography (HPLC) with diode array detection. The screening results showed that the extracted amount of active compounds (methylxantines and some polyphenols) varied greatly depending on the used solvent. DES choline chloride:oxalic acid was the most suitable solvent for the extraction of active compounds from CBS. The DES extraction was then coupled with microwave assisted extraction (MAE), for the comparison and the optimisation was performed by response surface methodology (RSM). Antioxidant activity was also determined using DPPH method. The statistical results showed that water content significantly influenced the extraction of targeted active compounds from CBS, while extraction time and temperature did not show statistically significant influence. The study demonstrated how extraction using DES and microwaves could be of a great importance in the future trends of green chemistry for the production of CBS extracts rich in bioactive compounds.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA