Naslov
Normal human kidney development: expression and localisation of DAB1 and Reelin

Autori
Racetin, Anita ; Jurić, Marija ; Lozić, Mirela ; Kosović, Ivona ; Šolić, Ivana ; Filipović, Natalija ; Saraga-Babić, Mirna ; Vukojević, Katarina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Pediatric Nephrology / - , 2019, 1842-1843

Skup
18th Congress of the International Pediatric Nephrology Association

Mjesto i datum
Venecija, Italija, 17.10.2019. - 21.10.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
DAB1 ; REELIN ; human embryo ; kidney ; immunofluorescence ; development

Sažetak
Background: The aim of this study was to explore spatial and temporal expression patterns of DAB1 and REELIN proteins in the tissues of healthy human kidneys to determine their possible roles during normal kidney development and in postnatal period. Methods: DAB1 and REELIN expression was examined in the paraffin sections of human fetal kidneys between the 13/14th and 38th developmental weeks and in postnatal kidney tissues of 1.5 and 7year old individuals by double immunofluorescence staining. Kidney structures were analyzed by ImageJ software: proximal convoluted tubules (PCT), distal convoluted tubules (DCT) and glomeruli (G). The data were analyzed by Kruskal Wallis test. Statistical significance was considered at P<0.05. Results: DAB1 and REELIN were expressed during fetal kidney development and postnatal period with specific spatial expression pattern and diverse fluorescence intensity. DAB1 was highly expressed with strong reactivity in DCT during fetal period, and variously in PCT and glomeruli. In all observed structures DAB1 was mostly expressed on cell membranes, usually at their apical parts. In postnatal kidneys DAB1expression decreased. In early fetal stages, REELIN was mostly spread throughout cytoplasm of PCT cells. In postnatal kidneys percentage of REELIN positive cells decreased dramatically in all observed structures. These two markers co- localized during early developmental stages, mostly in PCT, DCT and possibly in podocytes. Conclusion: Appearance of DAB1 and REELIN protein expression during human fetal kidney development confirms their potentially significant role in the morphogenesis or functional maturation of kidney structures. High DAB1 expression in DCT implies its regulatory role during development of those parts of kidney tubules. Decrease in expression of DAB1 and REELIN after birth implies gradual loss of their role in postnatal period. Poor co- localization of these two markers suggests their diverse role during kidney development.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA