Naslov
Combining Proteomics and Lipid Analysis to Unravel Confidor® Stress Response in Saccharomyces cerevisiae

Autori
Justinić, Iva ; Katić, Ana, Uršičić, Deni ; Ćurko-Cofek, Božena ; Blagović, Branka ; Čanadi Jurešić, Gordana

Izvornik
Environmental toxicology (1520-4081) 35 (2020), 3; 346-358

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Confidor ; stress response ; yeast ; lipid ; proteome

Sažetak
The yeast Saccharomyces cerevisiae is a useful model for studying the influence of different stress factors on eukaryotic cells. In this work we used the pesticide imidacloprid, in the Confidor® formulation, as the stress factor and analyzed its influence on the metabolic activity, proteome and lipid content and composition of Saccharomyces cerevisiae yeast. During the cultivation of yeast, the lowest recommended application dose of Confidor® (0.025 %, v/v) was added to the growth media and its influence on the mitochondria, cytosol with microsomes and the whole yeast cells was monitored. The results show that under the stress provoked by the toxic effects of Confidor®, yeast cells density significantly decreased and the percentage of metabolically disturbed cells significantly increased comparing with untreated control. Also, there was a downregulation of majority of glycolytic, gluconeogenesis and TCA cycle enzymes (Fba1, Adh1, Hxk2, Tal1, Tdh1, Tdh3, Eno1) thus providing enough acetyl-CoA for the lipid restructuring and accumulation mechanism since we have found the changes in the cell and mitochondrial lipid content and FA composition. This data suggest that lipids could be the molecules that orchestrate the answer of the cells in the stress response to the Confidor® treatment.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne biotehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA