Naslov
C type Lectin Receptor Expressions in Mature Synovial Tissue of Patients with Osteoarthritis

Autori
Ćurko-Cofek, Božena ; Legović, Dalen ; Šantić, Veljko ; Rukavina, Daniel ; Drvar, Vedrana ; Kehler, Tatjana ; Peršić, Viktor ; Laškarin, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
13th Annual Meeting of Croatian Physiological Society with International Participation / Drenjančević, Ines - : Craotian Physiological Society, 2019, 36-36

Skup
13th Annual Meeting of Croatian Physiological Society with International Participation

Mjesto i datum
Osijek, Hrvatska, 18.09.2019. - 19.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
C type lectin family receptors ; osteoarthritis ; synovial tissue ; NK cells ; CD8+T cells

Sažetak
Introduction: A family of C-type lectin receptors, including NKG2A, NKG2C and NK2D are expressed predominantly on the surface of NK cells and a subset of CD8+ T - cells. These receptors are efficient pattern-recognition receptors (PRRs) that interact with ligands via carbohydrate structures, leading to modulation of T and NK cell differentiation. Proteins that contain C-type lectin domains have a diverse range of functions, including cril-eell adhesion, Immune response to antigens and apoptosls. NKG2A inhibits, while NKG2C and NKG2D stimulate cytotoxic activity of NK cells. We investigated the presence arid tissue distribution of C-type lectin family receptors NKG2A, MKG2C, NKG2D in synovial tissue of patients with osteoarthritis (OA) in respect to tissue distribution Of T and NK cells. Material and Methods: Synovial tissue samples (no. 5) were obtained during total knee replacement surgery and embedded in paraffin cubes. Tissue sections were prepared as 5-6 urn thick cuts and labeled by single immunofluorescence using anti- NKG-2A (clone 131411, R&D: Systems, 0, 2 p.g/sample, dilution 1:100), anti-NKG-2C(clone 134522, R&D Systems, 2 jig/sample, dilution 1:100), anti-NKG-2D (clone 1D11, BD Pharmingen, 10 pg/sample, dilution 1:100), anti-CD8 (clone RPA-T8, BD Pharmingen, 20 ul/ slide) and' anti- CD 56 (clone: N901, IgGl ; Beckman Coulter), images were taken using an Olympus BX51 fluorescent microscope and Cell F program version 3.1 (Olymjjus, Tokyo, Japan). Microphotographs were randomly chosen Results: NKG2A and NKG2C were mostly scattered in sublining synovial tissue, resembling the CD8+ and CP56+ tissue distribution. NKG2D was present on the surface of synovial shoots and in the accumulations of lymphocytes. The distribution of this receptor resembles the most the distribution of synovial CD56+ cells. Conclusion: The presence of C-type lectin receptors NKG2A, NKG2C and NKG2D in synovial tissue of patients wjith'mature OA suggests that they might be involved in immunopathogenesis of the disease.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA