Naslov
Biocatalytic biodiesel synthesis with Burkholderia cepacia and Pseudomonas fluorescens lipase

Autori
Budžaki, Sandra ; Ostojčić, Marta ; Strelec, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 1st International Conference on Advanced Production and Processing publishes abstracts from the following fields: Innovative Food Science and Bioprocesses, Nutraceuticals and Pharmaceuticals, Sustainable Development, Chemical and Environmental Engineering, Materials Design and Applications, Petroleum Refining and Production / Vidović, Senka ; Jovicic, Mirjana ; Popovic, Ljiljana ; Sumic, Zdravko ; Hadnadjev, Milica ; Govedarica, Olga ; Vucetic, Snezana ; Bera, Oskar - Novi Sad : Tehnološki fakultet Univerziteta u Novom Sadu, 2019, 302-302

ISBN
978-86-6253-102-5

Skup
1st International Conference on Advanced Production and Processing (ICAPP 2019)

Mjesto i datum
Novi Sad, Srbija, 10.10.2019. - 11.10.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
lipase ; biodiesel ; Burkholderia cepacia ; Pseudomonas fluorescens

Sažetak
Biodiesel is a mixture of fatty acid methyl esters, usually produced by a transesterification reactions in the presence of sodium hydroxide as catalyst. However, this most often used industrial process for biodiesel synthesis has certain shortcomings. Thus, the use of new catalysts, and among them biocatalyst such as lipases are preferred. Lipases (EC 3.1.1.3.), triacylglycerol acylhydrolases, are enzymes of plant, animal or microbial origin, which are capable to perform efficient transesterification reactions in the presence of organic solvents. Although the use of lipases in biodiesel synthesis is well documented, there are few questions which still need to be resolved, and among it optimal conditions for biodiesel biocatalytic production. Namely, various temperatures and pH’s could be found reported for biodiesel production, which are in some instances different from optimal temperature and pH of lipases used in transesterification reaction. Moreover, determination of pH and temperature optimum for lipase activity has been performed by various substrates differing in their chemical structure and composition, which additionally complicates selection of optimal conditions. Therefore, the aim of this study was to determine pH and temperature optimum of Burkholderia cepacia and Pseudomonas fluorescens lipases using two chemically and structurally different substrates: olive oil and p-nitrophenyl palmitate, and to examine efficiency of biodiesel biocatalytic synthesis in batch reactor at determined optimal conditions for lipase activity. Results have shown that optimal conditions (pH and temperature) for lipase activity are differing regarding substrate used, and that those determined by olive oil are well in accordance with data obtained from manufacturer. Furthermore, during batch reactor biodiesel synthesis, greater amount of fatty acid methyl esters (FAME) were produced at optimal conditions determined with olive oil as lipase substrate, than with p-nitrophenyl palmitate. Data on the percentage of FAME in crude biodiesel has shown that Burkholderia cepacia lipase is more suitable for biodiesel synthesis, where more than 96.5% FAME were obtained after 24 hours of synthesis, in comparison to Pseudomonas fluorescens lipase with maximally 71.9% of FAME produced at optimal conditions. Obtained data indicates that greater amount of biodiesel can be produced if optimal conditions for lipase activity (pH and temperature optimum) are applied during batch reactor synthesis. However, optimal conditions for lipase activity should be determined with olive oil test as more reliable.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

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