Pregled bibliografske jedinice broj: 102597
Mechanism of murine cytomegalovirus entry into the cell nucleus
Mechanism of murine cytomegalovirus entry into the cell nucleus // American Journal of Reproductive Immunology / Gleicher, Norbert (ur.).
Kopenhagen: Munksgaard, 2001. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 102597 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Mechanism of murine cytomegalovirus entry into the cell nucleus
Autori
Kučić, Natalia ; Mahmutefendić, Hana ; Lučin, Pero
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
American Journal of Reproductive Immunology
/ Gleicher, Norbert - Kopenhagen : Munksgaard, 2001
Skup
VIII International Congress of Reproductive Immunology
Mjesto i datum
Opatija, Hrvatska, 02.07.2001. - 06.07.2001
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
-
Sažetak
Problem Immediately after the murine cytomegalovirus (MCMV) infection of fibroblasts the phpsphpprotein pp89 (IE), which is the product of immediate-early phase of viral replication is expressed in nucleus. The protein of the early phase of viral replication (E1) is expressed afterwards. Both viral proteins could be detected in cell nucleus 2-3 hrs post infection and be used as indicators of entry of MCMV components into this compartment. Methods of Study In order to investigate the mechanism of the MCMV transport through the cell and viral DNA transport into the cell nucleus the inhibitors of endocytosis (both clathrine and caveolar), vesicular transport and cytoskeletal function were used. The IE and E1 proteins were detected by immunofluorescence and Western blott analysis. Results The inhibitors of endocytosis and vesicular transport as well as depolimerizators of microtubules did not completely inhibit the viral entry into the cell nucleus. The entire block was achieved using the H7 [1-(4-Isoquinolinesulfonyl)-2-methyl-piperazine dihidrochloride], an inhibitor of protein kinase C (PKC) activation. Conclusions These results indicate that the mechanisms of clathrine and caveolar endocytosis are not used for viral entry into the cell. Othervise, for the transport through the cell and for the entry into the nucleus the processes of cytoskeletal phosphorylation are required. Supported by grant No 062006 from the Ministry of Science and Technology, Republic of Croatia.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
