Naslov
Chromatin Remodeling at the Yeast PHO5 Promoter: Correlating In Vivo Promoter Occupancy with Cofactor Requirement

Autori
Reinke, Hans ; Barbarić, Slobodan ; Korber, Philipp ; Hörz Wolfram

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Gene Transcription in Yeast, EuroConference on Signal Transduction, Transcriptional regulation and Chromatin Structure. / - Castelvecchio Pascoli, 2002

Skup
Gene Transcription in Yeast, EuroConference on Signal Transduction, Transcriptional regulation and Chromatin Structure

Mjesto i datum
Castelvecchio Pascoli, Italija, 31.05.2002. - 05.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chromatin remodeling; PHO5 promoter

Sažetak
Under conditions of phosphate starvation the PHO5 promoter of S. cervisiae undergoes a striking chromatin transition, whereby a chromatin microdomain consisting of four positioned nucleosomes is completely disrupet ( Svaren and Hörz, 1997). How this remodeling event is achieved is not yet understood. It is assumed that Pho4, the main transcriptional activator of PHO5, recruits transcriptional coactivators to the promoter including ATP-dependent remodeling machines as well as histone acetyltransferases. Although Gcn5 is not required for full activation of PHO5 the rate of PHO5 activation is greatly decreased in its absence (Barbaric et al., 2001). This delay in transcriptional activation is specifically due to slow chromatin remodeling of the PHO5 promoter, whereas the transmission of the phosphate starvation signal to the PHO5 promoter progresses at a normal rate. A role in affecting the rate of chromatin remodeling seems to be a more general property of chromatin modifying activities because a similar delay in PHO5 activation is observed when Snf2 is absent. Again the final extent of activation and chromatin remodeling is very similar to the level seen in a wt strain. We have asked the question, which cofactors are recruited at what time to the PHO5 promoter upon inductoin. Chromatin immunoprecipitation (ChlP) analysis employing tagged versions of the Pho4, Ada2 and Snf2 proteins was used to determine the kinetics with which these factors bind to the promoter. Data on how the recruitment of one coactivator affects the recruitment ot others is presented. The contribution and significance of SAGA and of histone acetylation for chromatin remodeling at the PHO5 promoter is discussed. References: Barbaric, S., Walker, J., Schmid, A., Svejstrup, J.Q. and Hörz, W. (2001). Increasing the rate of chromatin remodeling and gene activation - a novel role for the histone acetyltransferase Gcn5. EMBO J., 20, 4944-51. Svaren, J. and Hörz, W. (1997). Transcription factors vs nucleosomes: regulation of the PHO5 promoter in yeast. Trends Biochem Sci. 22, 93-97.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA