Naslov
Immunogold labelling of vitamin D receptor and ultrastructural characterisation of metabolizing enzymes in lipid droplets in the rat liver

Autori
Filipović, Natalija ; Bočina, Ivana ; Restović, Ivana ; Kević, Nives ; Grobe, Maximilian, Kretzschmar, Genia ; Mašek, Tomislav ; Vitlov Uljević, Marija ; Vukojević, Katarina ; Saraga- Babić, Mirna ; Vuica, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings from the 14th Multinational Congress on Microscopy / Grbović Novaković, Jasmina ; Nestorović, Nataša ; Rajnović, Dragan - Beograd : University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia Serbian Society for Microscopy, Serbia, 2019, 281-282

ISBN
978-86-80335-11-7

Skup
14th Multinational Congress on Microscopy (MCM 2019)

Mjesto i datum
Beograd, Srbija, 15.09.2019. - 20.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
hepatic steatosis, lipid droplets, vitamin D, VDR, 1α-hydroxylase, CYP 24

Sažetak
Vitamin D is a secosteroid with multiple actions throughout the body, usually obtained from diet, dietary supplements and exposure to sun lights. Its actions are mediated via its nuclear receptor, the vitamin D receptor (VDR). There are hard evidences that correlate vitamin D deficiency with liver lipid metabolism disturbances, but the mechanism of this action is still unknown. In our previous work, we localized the accumulation of VDR in membrane of the lipid droplets (LDs) in hepatocytes. In this study, we applied the transmission electron microscopy (TEM) by using a long-term (6 months) high sucrose intake rat model for research of the hepatic lipid accumulation. In addition to the VDR, vitamin D metabolizing enzymes 1α-hydroxylase and CYP 24 were found to be associated with the membrane of the LDs. A light-microscopy data revealed significant increase in expression of VDR and CYP 24 in liver of high-sucrose treated rats in comparison to controls. These results provide a new insight in the possible relation of vitamin D signalling system with LD morphology and function and with the lipid metabolism in general.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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