Naslov
Effects of sterigmatocystin on antioxidative enzymes and expression of Hsps in male Wistar rats

Autori
Peraica, Maja ; Rašić, Dubravka ; Hulina Tomašković, Andrea ; Micek, Vedran ; Jakšić, Daniela ; Rumora, Lada ; Šegvić Klarić, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Toxicology Letters / Dekant, Wolfgang - : Elsevier, 2019, S71-S72

Skup
55th Congress of the European Societies of Toxicology (EUROTOX 2019)

Mjesto i datum
Helsinki, Finska, 08.09.2019. - 11.09.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Catalase ; Glutathione peroxidase ; Heat shock proteins ; Superoxide dismutase
(catalase ; glutathione peroxidase ; heat shock proteins ; superoxide dismutase)

Sažetak
Sterigmatocystin (STC), a precursor of aflatoxin B1, is mycotoxin which International Agency for Research on Cancer evaluated as Group 2B. STC was isolated in 1954 from Aspergillus versicolor and afterwards from other Aspergillus species producing it as aflatoxin B1 precursor or as final mycotoxin. Due to its structural similarity to carcinogen AFB1, STC carcinogenic potential was studied much more than its toxicity. The aim of this study was to evaluate the effect of STC on antioxidative enzymes and heat shock proteins (Hsp 70 and Hsp27) as parameters of oxidative stress. Male Wistar rats (N=5 per group) were treated with single oral STC doses of 1/16, 1/8, and 1/4 of LD50 (10, 20 and 40 mg kg-1 b.w.). Control group was treated with corn oil which was used as STC vehicle. Catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity in plasma, kidneys and liver were measured on a plate reader using commercial kits. Expressions of heat shock proteins Hsp70 i Hsp27 were measured in kidneys and liver using Western blot methods. CAT activity did not change in organs of treated animals. GPx activity was significantly lower in liver of rats treated with 1/8 and 1/4 LD50. Activity of SOD was significantly increased in kidneys of rats given 1/16 and 1/4 LD50. Expression of Hsp70 was significantly increased in liver samples of rats treated with 1/16 LD50 ; in kidneys the same dose augmented expression of Hsp70 but without significant difference to control group. Highest STC dose returned Hsp70 expression to control values. Expression of Hsp27 in liver and kidneys was not significantly changed at any STC dose. Taken together our findings suggest that acute oral exposure to STC causes oxidative stress both in liver and kidneys.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti, Javno zdravstvo i zdravstvena zaštita, Farmacija

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