Naslov
Spatially clustered loci with multiple enhancers are frequent targets of HIV-1 integration

Autori
Lucic, Bojana ; Chen, Heng-Chang ; Kuzman, Maja ; Zorita, Eduard ; Wegner, Julia ; Minneker, Vera ; Wang, Wei ; Fronza, Raffaele ; Laufs, Stefanie ; Schmidt, Manfred ; Stadhouders, Ralph ; Roukos, Vassilis ; Vlahovicek, Kristian ; Filion, Guillaume J. ; Lusic, Marina

Izvornik
Nature Communications (2041-1723) 10 (2019), 1; 4059, 12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
HIV-1 integration ; multiple enhancers ; spatial compartmentalization

Sažetak
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained unclear. Here we show that recurrently targeted genes are proximal to super-enhancer genomic elements and that they cluster in specific spatial compartments of the T cell nucleus. We further show that these gene clusters acquire their location during the activation of T cells. The clustering of these genes along with their transcriptional activity are the major determinants of HIV-1 integration in T cells. Our results provide evidence of the relevance of the spatial compartmentalization of the genome for HIV-1 integration, thus further strengthening the role of nuclear architecture in viral infection.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA