Naslov
Specificity and sensitivity of two Hepatitis C virus antibodies confirmatory immunoblot assays in Croatian blood donors, 2013-2015

Autori
Miletić, Manuela ; Struck, Friedhelm ; Naewiger, Michaela ; Brill, Marianne ; Reichhuber, Christine ; Böcher, Oliver ; Soutschek, Erwin ; Stošić Đurić, Darka ; Jukić, Irena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Biochemia Medica 2018 ; 28(Suppl 1):S1-S223 / - , 2018, S181-S182

Skup
9. kongres hrvatskog društva za medicinsku biokemiju i laboratorijsku medicinu (HDMBLM)

Mjesto i datum
Zagreb, Hrvatska, 09.05.2018. - 12.05.2018

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
immunoblot tests, blood donor testing

Sažetak
Introduction: Anti-HCV screening of blood donors (BD) is manadatory in Croatia. Non-specific or false biological reactivities, despite execellent sensitivity and specificity of anti-HCV (immunoassays, IAs) assays, are present. A confirmation testing algorithm is required, including immunoblot (IB) assay/s, to clarify IA's reactivity. We analysed two different confirmatory IB anti-HCV assays, recomLine HCV IgG and INNO-LIA HCV Score, to determine their sensitivity and specificity. Materials and Methods: From 2013 to 2015 in Croatia were tested 183.072, 183.410 and 195.534 blood donations - per year, respectively. Screening anti-HCV tests used are mainly Abbott's IAs, Prism and Architect. Each initially reactive result is retested in duplicate and repeatedly reactive (RR) results/samples are sent to CITM for confirmatory testing. Overall specificity of anti-HCV for each year was equal, 99.91%. For confirmation testing we got 181, 164 and 190 BD's donation RR samples and 155, 88 and 101 BD's control samples, per year respectively. Used were IAs: Bio-Rad Anti-HCV v.2, Bio-Rad Monolisa HCV Ag-Ab Ultra and Vidas anti-HCV, and IBs: recomLine HCV IgG (Mikrogen GmbH, Neuried, Germany), which was performed on DynaBlot, and INNO-LIA HCV Score (Fujirebio Europe N.V., Belgium), performed on Auto-LIA 48 analyzer. All of the testing was done according to the producer's instrustions. Results: Confirmed postitive were 14, 7 and 7 BD in 2013, 2014 and 2015, respectively. Specificity found was significantly higher for recomLine HCV IgG , 78.7%, and 59.6% for INNO-LIA HCV Score. Sensitivity found for recomLine HCV IgG was 79.0% and for INNO-LIA HCV Score test 75.8%. Conclusion: The results of this study indicate that IB recomLine HCV IgG has better specificity then INNO-LIA HCV Score test, with similar sensitivity. Good specificity of the IB test is mandatory to determine false positive reactivities in the screening and reentry of blood donors.

Izvorni jezik
Engleski

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