Naslov
The development of optimal process design of lactol synthesis catalyzed by 2-deoxyribose-5-phosphate aldolase

Autori
Švarc, Anera ; Vasić-Rački, Đurđa ; Findrik Blažević, Zvjezdana ; Vrsalović Presečki, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Izvornik
Biocatalysis as a Key Enabling Technology / - , 2017

Skup
Biocatalysis as a Key Enabling Technology, H2020 Workshop 2017

Mjesto i datum
Siena, Italija, 03.10.2017. - 06.10.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
optimization ; DERA ; statin ; mathematical modelling

Sažetak
The examined process is a two-step aldol reaction catalyzed by the genetically modified 2- deoxyribose-5-phosphate aldolase (DERA, E.C. 4.1.2.4) in which the acceptor substrate chloroacetaldehyde (1) reacts with the donor substrate acetaldehyde (2) and gives an intermediate aldehyde (3) that subsequently reacts with a second donor molecule of 2 and results with the key product lactol (4), a valuable chiral synthon for the common statin- type side chain of a cholesterol-lowering drug. The main drawbacks of this process are the enzyme deactivation in the presence of substrates 1 and 2 and the negative impact of product 3 on the enzyme’s stability. Therefore it is essential to develop an effective process for large-scale production, and thus an efficient reactor mode, to diminish the present enzyme deactivation. To successfully achieve it, modeling of reaction kinetics plays an important role. Based on experimentally determined kinetic parameters of the DERA-catalyzed aldol reaction mathematical models of different reactor modes were developed. The models include the negative impact of all aldehydes on the enzyme stability, which were experimentally determined by measuring the enzyme deactivation in different aldehyde (1, 2, or 3) concentrations. In this study three different reactor modes were examined: batch, fed-batch and continuous membrane reactor, and by changing the initial conditions of those, the model simulations were obtained. The optimal process design was proposed based on calculated productivity and biocatalyst consumption.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo

POVEZANOST RADA