Naslov
Stability and kinetic characterization of 2- deoxyribose-5-phosphate aldolase in the synthesis of a statin intermediate

Autori
Švarc, Anera ; Sudar, Martina ; Vrsalović Presečki, Ana ; Vasić-Rački, Đurđa ; Findrik Blažević, Zvjezdana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Izvornik
Systems Biocatalysis 2016 / - , 2016

Skup
Systems Biocatalysis, COST Training School

Mjesto i datum
Siena, Italija, 27.04.2016. - 30.04.2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biocatalysis ; statins ; DERA

Sažetak
Since the enzyme activity decreases when exposed to an unfavorable environment, the enzyme stability is a crucial factor to determine the successful commercial application of the enzyme. Thus, the effects of temperature and different buffers at various pH on DERA activity and stability were investigated as well as the influence of different chloroacetaldehyde and acetaldehyde concentrations. The DERA enzyme was kinetically characterized in the reaction aldol reaction of AA with CAA and a mathematical model for the 6-chloro-3, 5-dihydroxyhexanal production in the batch reactor was proposed. In order to examine te enzyme operating stability during a prolonged use, a repetitive batch reaction with acetaldehyde and chloroacetaldehyde as substrates was performed.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo

POVEZANOST RADA