Naslov
HAB species of the northern Adriatic (Mediterranean Sea) recorded using metabarcoding

Autori
Baričević, Ana ; Chardon, Cécile ; Marić Pfannkuchen, Daniela ; Kužat, Nataša ; Vasselon, Valentin ; Smodlaka Tanković, Mirta ; Rimet, Frédéric ; Bouchez, Agnès ; Pfannkuchen, Martin

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
ICHA 2018 Abstract book / Hess, Philip - Nantes : The International Society for the Study of Harmful Algae (ISSHA), 2018, 453-453

Skup
18th International Conferences on Harmful Algae (ICHA 2018)

Mjesto i datum
Nantes, Francuska, 21.10.2018. - 26.10.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Adriatic Sea ; diatoms ; metabarcoding

Sažetak
The northern Adriatic Sea has a long-term monitoring programme and the well-known diversity of phytoplankton. Still, species identification is routinely based only on morphological analysis using light microscopy (LM) what may be limiting for the taxonomic determination of many phytoplankton species. To complement LM for species identification, diversity of four long-term monitoring northern Adriatic stations was for the first time assessed using metabarcoding together with LM. Special focus of the study was on HAB species detection since several genera containing harmful algae are virtually indeterminable during routine LM and other genera are presumed to be present in only marginal abundances. In the 18S-V4 and rbcL dataset obtained from all the stations, ten microphytoplankton genera (Alexandrium, Amphidinium, Azadinium, Gymnodinium, Gyrodinium, Heterocapsa, Karlodinium, Protoceratium, Pseudo-nitzschia and Symbiodinium) with known HAB and toxic species representatives were identified. Some genera (Azadinium, Heterocapsa, Karlodinium and Symbiodinium) represent first records for the region studied. Genera Gyrodinium and Alexandrium had the highest OTU counts associated to them. Very good database (Silva, GenBank, DinoREF) representation of the genus Alexandrium enabled species level identification of A. minutum and A. pseudogonyaulax. Shared HAB genera detected by both metabarcoding and LM were of 50%. Abundance and incidence patterns of the genera previously included in long-term microscopy monitoring matched the metabarcoding data. With the more profound approach in the HAB species identification then microscopy alone, metabarcoding managed to add significant knowledge to harmful algae diversity of the northern Adriatic Sea. Our study therefore confirms that metabarcoding represents a promising tool for HAB monitoring.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

Napomena
COST DNA aquanet

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