Naslov
Diversity of marine Cyanobacteria in coastal waters and sediments in central and southern eastern Adriatic sea

Autori
Kolda, Anamarija ; Ljubešić, Zrinka ; Mucko, Maja ; Žunić, Jakov ; Gavrilović, Ana ; Jug-Dujaković, Jurica ; Pikelj, Kristina ; Šoštarić Vulić, Zvjezdana ; Kapetanović, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Seventh European Phycological Congress, European Journal of Phycology, vol. 54, sup. 1 / Ljubešić, Zrinka ; Bosak, Sunčica - : European Journal of Phycology, 2019, 190-190

Skup
7th European Phycological Congress

Mjesto i datum
Zagreb, Hrvatska, 25.08.2019. - 30.08.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Cyanobacteria ; Adriatic sea ; water column ; sediment ; NGS

Sažetak
Mediterranean Sea, characterized by extreme oligotrophy and bathymetrically divided into northern, central and the southern Adriatic basin. The aims of the study were to determine Cyanobacteria diversity within the microbial community in the water and sediment, identify the environmental factors in coastal waters of the sites and connect these findings to get insight into marine cyanobacterial ecology. Sampling was conducted in all four seasons during 2017 at two sampling points in coastal eastern Adriatic Sea. First sampling point in central Adriatic is oligotrophic, in a clove under the influences of the open sea, and second in southern Adriatic is moderately eutrophic, in the bay influenced by freshwater riverin input. Water samples were taken with Niskin sampler throughout the water column (maximum depth 20 m), while sediment samples were taken by the diver. Physico-chemical properties of the seawater were measured in situ (Secchi depth, turbidity, conductivity, temperature, salinity, oxygen concentrations and saturations, pH, TDS and ORP) and concentrations of N, P, Si and SiO2 were measured in the laboratory. Total bacterial DNA was extracted from water and sediment and the relative abundance and composition of 16S rRNA phylogenetic marker was determined using 16S rRNA (V1–V3 region) Illumina MiSeq. Bioinformatics and statistical analysis were performed using QIIME2 and RStudio. Water samples in both sampling sites were dominated by Synechococcus and Prochlorococcus, with many reads determined as “Other” and/or assigned as cyanobacterial chloroplast 16S rRNA. These reads had to be filtered after the assign taxonomy step, checked in NCBI database and a phylogenetic tree was constructed to get more precise taxonomical determination. The same procedure was conducted for sediment samples, which harboured great abundances of chloroplast reads, but also Pleurocapsa and uncultured taxa at both sampling sites, while Lyngbya and Oscillatoria were detected only at the second location.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA