Naslov
Phospholipid nanovesicles for the improved topical treatment of genital infections

Autori
Vanić, Željka ; Rukavina, Zora ; Manner, Suvi ; Fallarero, Adyary ; Uzelac, Lidija ; Kralj, Marijeta ; Amidžić Klarić, Daniela ; Filipović-Grčić, Jelena ; Skalko-Basnet, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Global Experts Meeting on Frontiers in Nanomedicine & Drug Delivery, Nano Delivery 2019 / - , 2019, 18-18

Skup
Global Experts Meeting on Frontiers in Nanomedicine & Drug Delivery (Nano Delivery 2019)

Mjesto i datum
London, Ujedinjeno Kraljevstvo, 18.03.2019. - 20.03.2019

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Phospholipids ; Liposomes ; Genital infections ; Topical therapy

Sažetak
Background: Genital bacterial infections represent one of the most common medical problems with expanding economic burden for the healthcare system. The recommended treatment regimens comprise both oral and local administration of antibiotics. However, concerns of drug resistance and possible teratogenicity in pregnant woman as well as lack of efficient localized therapy impose the necessity for considering other modes to combat genital infections. Vaginal application of antibiotic-loaded phospholipid nanovesicles (liposomes) presents a promising strategy for the enhanced drug delivery to bacterial cells.1 Tuning the liposomal physical properties enable design of the liposomes with favored pharmacokinetic and pharmacodynamics drug profiles.2 Therefore, the aim of the present study was to develop an efficient, safe and stable liposome-based delivery nanosystem for the improved topical vaginal therapy. Methods: Several types of phospholipid nanovesicles differing in size, surface charge and bilayer elasticity, namely conventional (CLs), propylene glycol (PGLs) and deformable propylene glycol liposomes (DPGLs) encapsulating azithromycin (AZT) were prepared and evaluated for the physical properties, in vitro release and storage stability. The optimized preparations were tested for ex vivo permeability through porcine vaginal mucosa and in vitro antibacterial and anti-biofilm activities against several Escherichia coli strains, while their biocompatibilities were examined on the cervical HeLa cells. Results: Phospholipid composition, presence of monoacyl phospholipid and propylene glycol significantly influenced physical characteristics of liposomes, in vitro drug release profiles and stability during storage. Thus, the slowest AZT release was achieved by CLs, followed by PGLs and DPGLs. Negatively surface charged CL-3, PGL-2 and DPGL-2 significantly affected the retention of AZT on the vaginal surface and within tissue in comparison to control and were more effective against E. coli (both planktonic and biofilm forms). Conclusion: Even though all the tested liposomes were biocompatible with HeLa cells in vitro, CL-3 and PGL-2 were shown as the most promising liposomes for further investigations.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA