Naslov
Untargeted screening of the bound / free phenolic composition in tomato cultivars for industrial transformation

Autori
Rocchetti, Gabriele ; Senizza, Biancamaria ; Putnik, Predrag ; Bursać Kovačević, Danijela ; Barba, Francisco J., Trevisan, Marco ; Lucini, Luigi

Izvornik
Journal of the science of food and agriculture (0022-5142) 2019 (2019); 1-9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
bound phenolics ; polyphenols ; food metabolomics ; food quality ; in vitro antioxidant activity ; UHPLC-QTOF-MS

Sažetak
BACKGROUND:Tomato is one of the most important agricultural crops and it is characterized by awide bioactive compoundprofile. However, little information is reportedonits comprehensive polyphenolprofile. In thiswork, 13commercialtomato cultivars for industrial transformationwere screenedby ultra-high- pressure liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF-MS) for both free and bound phenolic profiles. Thereafter, the in vitro antioxidant activity of each cultivar was assessed by ferric reducing antioxidant power (FRAP) and oxygen radical absorbance activity (ORAC) assays. Multivariate statistics, i.e. orthogonal projection to latent structures discriminant analysis (OPLS-DA), were then used tomodel samples according to their distinct phenolic signatures, thus providing compounds that better discriminated between the distributions of the cultivars that were considered. RESULTS: More than 350 phenolic compounds could be identified across the samples that were considered: flavonoids (such as flavones and flavanols), hydroxycinnamic acids, lignans, and lower-molecular-weight phenolics were the most frequently observed classes of phenolics in tomato berries. Anthocyanins were the most abundant class among bound phenolics (being highest in the Leader F1 and Defender F1 cultivars), followed by tyrosols (mainly in Heinz cultivars). However, flavones and hydroxybenzoic acids were the most represented discriminant phenolics in the bound fraction. CONCLUSIONS: Untargeted metabolomics allowed significant differences in phenolic composition to be outlined across the tomato cultivars that were analyzed. Such differences were particularly evident regarding the free- to-bound phenolic ratio, hence allowing differences in the bioaccessibility of phenolics to be postulated.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

Napomena
Inozemne institucije koautora na radu:
Università Cattolica del Sacro Cuore, Piacenza,
Italy ;
Faculty of Pharmacy, Universitat de València,
Spain

POVEZANOST RADA