Naslov
Retrospective study of canine infectious haemolytic anaemia cases reveals the importance of molecular investigation in accurate postmortal diagnostic protocols

Autori
Beck, Ana ; Huber, Doroteja ; Antolić, Maja ; Anzulović, Željka ; Reil, Irena ; Polkinghorne, Adam ; Baneth, Gad ; Beck, Relja

Izvornik
Comparative immunology, microbiology and infectious diseases (0147-9571) 65 (2019); 81-87

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
anaemia ; dogs ; retrospective study ; tick borne pathogens ; leptospira interrogans

Sažetak
Infectious haemolytic anaemia (IHA) in dogs share similar clinical signs including fever, lethargy, icterus, pa-leness of mucous membranes and splenomegaly. Postmortalfindings are similar and, without additional diag-nostic methods, an accurate aetiological diagnosis is difficult to achieve. In order to investigate causes of lethalIHA in Croatian dogs, we performed a retrospective study on archived formalin-fixed, paraffin-embedded tissueblocks (FFPEB) from dogs that died due to haemolytic crisis, using microscopic and molecular diagnostic tools todetermine the aetiological cause of disease. Molecular analysis was performed on kidney, lung, myocardium andspleen on FFPEB from all dogs. The originally stated aetiological diagnosis ofB. canisor leptospirosis wasconfirmed in only 53% of the dogs. PCR and sequencing revealed that, in addition to the expected pathogens, B.canisandLeptospira interrogans, the presence of previously undiagnosed“new”pathogens causing anaemia in- cludingCandidatusNeoehrlichia mikurensis andAnaplasma phagocytophilum. Furthermore, Theileria capreoliwasdetected for thefirst time in a dog with postmortal descriptions of lesions. Intensive extravascular hemolysis wasnoticeable as jaundice of the mucosa, subcutis and fat tissue, green or yellow discoloration of renal parenchymacaused by bilirubin excretion in the renal tubules and bile accumulation within the liver in 90% of the dogs. Thiswork highlights the value of molecular diagnostics to complement traditional ante-mortem and post- mortemdiagnostic protocols for the aetiological diagnosis of pathogens associated with IHA.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA