Naslov
Structural characterisation of the novel exopolysaccharide biosynthesised by potential probiotic strain Lactobacillus fermentum D12

Autori
Butorac, K., Bellich, B., Banić, M., Leboš-Pavunc, A., Novak, J., Durgo, K., Cescutti, P., Rizzo, R., Zjalić, S., Šušković, J., Kos, B.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, ostalo, ostalo

Skup
1st International Meeting One Health in Zadar. Global approach to Global Health

Mjesto i datum
Zadar, Hrvatska, 03.06.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
Lactobacillus, exopolysaccharides, probiotic activity, structural characterization

Sažetak
Lactobacillus fermentum D12, biosynthesize exopolysaccharides, released, in a large amounts, in MRS broth supplemented with glucose. High Performance Size Exclusion Chromatography (HPSEC), 1H-NMR, GC and GC-MS analysis revealed that this strain produces three different types of EPOLs ; the one homopolysaccharide (HoPOL) of a molecular weight of 400 kDa and two different low molecular weight heteropolysaccharides (HePOLs) of less than 2 kDa. 2D-NMR spectroscopy analysis revealed that HoEPOL, with the highest molecular mass, is composed of repeating units of D-glucose linked by an α-1, 4-glycosidic bond, where 20 % of the glucose subunits is acetylated at C-3. Further chromatographic analyses and NMR experiments showed that the each HePOL contained mannose, glucose and galactose in an averaged relative molar ratio of 1.78:0.87:1 and 6.38:1.6:1, respectively. Since a probiotic strain survival in rigorous gastrointestinal (GI) conditions is the first probiotic selection criterion to be met and with the respect to efficient survival of D12 strain in GIT in vitro (bacteria counts ≥106 CFU ml-1), the potential probiotic role of Lb. fermentum D12 was evaluated. Also, sensitivity to different antibiotics, minimum inhibitory concentrations (MICs), antagonistic activity and analysis of fermentation of different carbohydrates using API 50 CHL media of this potential probiotic strain was assessed.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA