Naslov
Influence of repeated pulsed pesticide exposures on the biochemical biomarkers in Lemna minor

Autori
Moslavac, Marko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SEFS11 Abstract book / Sertić Perić, Mirela ; Miliša, Marko ; Gračan, Romana ; Ivković, Marija ; Buj, Ivana ; Mičetić Stanković, Vlatka - Zagreb : Hrvatsko udruženje slatkovodnih ekologa (HUSEk), 2019, 397-397

Skup
11th Symposium for European Freshwater Sciences (SEFS11)

Mjesto i datum
Zagreb, Hrvatska, 30.06.2019. - 05.07.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Pesticides ; Pulsed exposures ; Lemna minor ; Biomarkers ; Oxidative stress

Sažetak
The usage of pesticides in intensive agricultural production has been increasing, and among them herbicides are the most commonly used agrochemicals. Isoproturon is a highly selective phenylurea derived systemic herbicide. It is widely applied to prevent the development of annual grasses and broad-leaved weeds in agricultural fields. Isoproturon may enter the aquatic environment by a number of various pathways. Thus, it is released to aquatic ecosystems in a series of sequential pulses, which fluctuate considerably in continuance and concentration. Laboratory ecotoxicity tests employ constant exposure concentrations over defined duration and therefore may not realistically reflect the effects of certain xenobiotic on non-target organisms. The overall aim of this study was to evaluate the effects of isoproturon on molecular biomarkers and the occurrence of oxidative stress in the aquatic plant Lemna minor. Common duckweed plants were treated with 50, 100, 150 and 200 μg L-1 of isoproturon added in Steinberg’s nutrient solution during 21 days. The effects of isoproturon were assessed by measuring the activities of ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPX) and total protein concentration. Additionally, the effects of pulse isoproturon exposures (48 h of herbicide treatment, as well as 5 days of post-exposure period in pure nutrient solution) were studied. Enzymatic activities as ecotoxicological biomarkers, indicated that isoproturon treated plants were under oxidative stress. Results from studies that include multiple pulse exposures, may contribute to the better improvement of standardized ecotoxicity tests.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA