Naslov
Effects of micro-CT Scanning When Assessing Bone Density Associated with mtDNA Yield

Autori
Marino, Grace ; Ryan, Timothy M. ; Bašić, Željana ; Kruzic, Ivana ; Holland, Mitchell M.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
11th ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine / Primorac, Dragan ; Schanfield, Moses ; Vuk-Pavlović, Stanimir ; Kayser, Manfred ; Ördög, Tamás - Zagreb : International Society for Applied Biological Sciences (ISABS), 2019, 311-311

ISBN
987-953-57695-3-8

Skup
11th ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine

Mjesto i datum
Split, Hrvatska, 17.06.2019. - 22.06.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Micro-CT Scanning ; Bone denisty ; Ancient DNA damage

Sažetak
Mitochondrial (mt) DNA analysis is a routine method used in the identification of human skeletal remains. In order to successfully obtain DNA profiles and identify individuals associated with skeletal remains, scientists must be able to extract sufficient amounts of DNA. A factor in extracting larger DNA yields is the part of the bone that is sampled. This project will employ a micro-CT scanner in a novel way to determine the region of a bone that produces the greatest yield of mtDNA in relation to bone density. In the first phase of this study, the main goal was to assess DNA damage generated as a result of radiation of bone samples during the micro-CT scanning process, measured by evaluating mtDNA yields and degradation, and by massively parallel sequencing (MPS). Six samplings were taken from each of six femur bones from grave sites located in the Dominican monastery of St. Katarina in Split and dated to the 16th to 19th centuries. Half of the cuttings were scanned, and thus, exposed to radiation, while the remaining cuttings were not scanned. The six cuttings from each femur went through DNA extraction, mtDNA quantification and degradation analysis, amplification of the mtgenome using the PowerSeqTM Mito, Whole system (Promega), and MPS analysis on the MiSeq (Illumina). The focus of the second phase of the study will be to determine if there is a correlation between bone density and mtDNA yield. Cuttings will be taken from cortical and cancellous regions of samples that are representative of multiple bones of the human body. All of the samples will be scanned, and the relative density of each cutting will be determined using a bone density standard. The mtDNA yield of the samples will be compared and recommendations will be made for bone sampling methods. Findings from the first phase of the study, including relative mtDNA yields, degradation assessments, and MPS damage patterns across the entire mtgenome, will be presented.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Etnologija i antropologija

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