Naslov
Degradation of methadone in model biodegradation and ozonation experiments: elimination kinetics and transformation products

Autori
Terzić, Senka ; Kostanjevečki, Petra ; Sviličić Petrić, Ines ; Ćurko, Josip ; Matošić, Marin ; Ahel, Marijan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, ostalo

Skup
15th International Symposium on Persistent Toxic Substances (ISPTS)

Mjesto i datum
Basel, Švicarska, 06.11.2018. - 11.11.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
Methadone ; Ozonation ; UPLC/Q-Tof ; Biodegradation ; Transformation products

Sažetak
The aim of this work was to examine the removal of a difficult-to-degrade opioid analgesic methadone (MTHD) in biodegradation and ozonation studies. The biodegradation study was performed by activated sludge culture adapted to high concentration of methadone (10 mg/L) and included determination of elimination kinetics of the parent compound, taxonomic characterization of microbial culture based on the 16S rRNA gene amplicon sequencing and identification of biotransformation products. The ozonation experiments were performed in three different matrices, including pure water, phosphate buffer and secondary effluent from the Central wastewater treatment plant of the city of Zagreb. The MTHD removal rate was systematically studied as a function of ozone concentration, pH and matrix. The determination of the remaining MTHD concentrations as well as the identification of transformation products formed during the biodegradation and ozonation experiments was performed by ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry. Depending on the experimental conditions, the elimination efficiency of methadone (10 mg/L) in biodegradation experiments varied from 9% to 93% with the corresponding half-lives (t1/2) from 11.4 days and 1.5 days. A significantly faster elimination (t1/2 from 1.5 days to 4.1 days) was achieved at cometabolic conditions, using glucose-containing media, as compared to the experiments with MTHD as a single organic carbon source (t1/2 = 11.4 days). Moreover, higher biotransformation rate was associated with the additional supplementation of ammonia, revealing a possible impact of nitrogen availability on the transformation rate. The elimination of the parent compound was associated with the formation of 3 different TPs, characterized by m/z 278, 264 and 324. Two of these TPs were found to be identical to the main human metabolites of MTHD, 2-Ethylidene-1, 5- dimethyl-3, 3-diphenylpyrrolidine (EDDP) and 2- ethyl-5-methyl-3, 3- diphenyl-1- pyrroline (EMDP). EDDP represented over 90% of the total TP concentration at the end of experiment. Ozonation at an ozone dosage of 0.05- 0.5 mg/L completely removed MTHD in less than 5 min in pure water and phosphate buffer solution, providing that pH of the ozonation medium was higher than 7. The elimination of MTHD was significantly slowed down at acidic conditions, which indicated the importance of the amino group deprotonation for an efficient reaction with ozone. MTHD elimination in secondary effluent was much slower (t1/2 = 1.72 min) than in organic- free water matrices (t1/2 = 0.40 - 0.8 min). The removal of MTHD in ozonation experiment was associated with a formation of three transformation products, characterized by m/z 278, m/z 294 and m/z 326, which were identified as EDDP, EDDP N-oxide and MTHD N-oxide, respectively. Both degradation approaches are promising for the development of viable technologies aimed at the removal of methadone from heavily loaded wastewater, however the final assessment of the degradation outcome by both methods warrants a more detailed ecotoxicological evaluation of the TPs formed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Kemijsko inženjerstvo

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