Naslov
Massively parallel sequencing (MPS) technology in forensic genetics

Autori
Korolija, Marina ; Sukser, Viktorija ; Rožić, Sara

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Program and Abstracts of the Eleventh ISABS Conference on Forensic and Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine / - , 2019, 331-331

Skup
7th Croatian Congress of Human Genetics

Mjesto i datum
Split, Hrvatska, 16.06.2019

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
massively parallel sequencing, forensic genetics

Sažetak
Massively parallel sequencing (MPS) technology provided potential to get fast, cost-effective and comprehensive insight into individual genomes of all living organisms. Accessibility of huge amount of sequencing data enabled discovery of new genetic markers relevant for unique, intraspecific characterization of species members. Besides many other applications, an explosion of new findings on individual differences became extremely interesting for forensic genetics. After all, the main goal of biological trace analysis in forensics is to maximize probability of individualization and to assess relationships between participants involved in criminal case. Traditional method of forensic DNA typing, capillary electrophoresis of short tandem repeats (CE-STR), has been in use for decades. Its extreme efficiency in identification has been proved countless times. However, it seems that the time for taking over by new, advanced, more powerful technology has finally come. Not only does MPS increase discrimination power of all inferences of identity, ancestry and kinship produced by CE-STR analysis, it also brings a spectrum of new forensic applications that are completely beyond the grasp of CE-STR. It is mainly so because unlike length-based method, information like phenotype, age, biogeographic origin etc. are based on many, simultaneously analyzed sequence variations. In our research, striving towards implementation of MPS into routine forensic practice, we employ MiSeqTM FGx system with ForenSeqTM DNA Signature Prep Kit (Illumina®) that enables simultaneous sequencing of 58 STR markers, as well as 174 single nucleotide polymorphysms relevant for identity, phenotype and ancestry inference in a single sample. Presently, many laboratories test MPS performances in many forensic aspects. Although some of applications might fail to prove useful for actual casework, only comprehensive research will accelerate final implementation, thus shaping the future of forensic genetics.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA