Naslov
Konzumacija jaja obogaćenih omega-3 masnim kiselinama smanjuje oksidativni stres u granulocitima zdravih muških ispitanika
(The Consumption of the Omega-3-Enriched Eggs Reduces an Oxidative Stress in Granulocytes of the Healthy Male Participants)

Autori
Kolobarić, Nikolina ; Mihalj, Martina ; Matić, Anita ; Stupin, Ana ; Barić, Lidija ; Kralik, Zlata ; Kralik, Gordana ; Drenjančević, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Young Scientists Conference BOOK OF ABSTRACTS / - , 2019, 12-12

Skup
2nd Young Scientist Days - Conference

Mjesto i datum
Osijek, Hrvatska, 23.05.2019. - 24.05.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
oksidativni stres ; antioksidativna aktivnost ; omega-3 jaja
(oxidative stress ; antioxidative activity ; omega‐3 eggs)

Sažetak
The study aimed to investigate the effects of an n-3 PUFA enriched egg consumption on the levels of an oxidative stress in human leukocyte subsets. Thirty‐three healthy participants (aged 20 to 26) were divided into two groups: the first group consumed the n‐3 PUFA enriched chicken eggs (OE ; n=18, 2 eggs/day, n‐3 PUFA=403.10 mg/day), while the second group consumed the regular chicken eggs (RE ; n=15 ; 2 eggs/day, n‐3 PUFA=74.63 mg/day) for three weeks. A peripheral leukocyte oxidative stress was measured by flow cytometry (BD FACS Canto II flow cytometer) with DCF‐DA (2’, 7’- dichlorofluorescin diacetate) assay at the beginning and at the end of the study. The levels of an intracellular ROS were assessed prior to and 30 minutes subsequent to a phorbol‐ 12‐ myristate‐13‐acetate (PMA) (1:1000 ; 50 µL)‐ mediated induction of the ROS production. The statistical analyses were performed using the Sigma Plot and the GraphPad Prism. A t‐ test was used for the differences among the groups, and an intra‐group difference were tested by a paired t‐test, where p<0.05 was considered significant. A study protocol and procedures conformed to the standards of the Helsinki Declaration and were approved by the Ethical Committee of the Faculty of Medicine, University of Osijek. The OE group exhibited significantly reduced levels of ROS upon a PMA stimulation in the male granulocytes (p=0.0442), in contrast to a significantly increased ROS production upon a PMA stimulation in the male and female monocytes from the OE respondents (p=0.0006 and p=0.0227, respectively). The OE men had a significantly lower level of oxidative stress in granulocytes prior to and upon a PMA stimulation (p=0.0366 and p=0.0049, respectively) if compared to the RE men. The OE men had the significantly lower levels of ROS in monocytes and lymphocytes if compared to the OE women (p<0.0001 and p=0.0153, respectively). An omega‐3 consumption reduced the levels of oxidative stress upon a PMA stimulation in the granulocytes of men, while it exerted an opposite effect on the monocytes of both sexes. The results suggest that the omega‐3 supplementation reduces an oxidative stress in granulocytes, probably by increasing a cellular antioxidative capacity.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
The work is supported by a grant to the Scientific Center of
Excellence for a Personalized Healthcare, Josip Juraj
Strossmayer University of Osijek, Croatia.

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