Aerobic biodegradation of tramadol by pre- adapted activated sludge culture: Cometabolic transformations and bacterial community changes during enrichment

Kostanjevečki, Petra; Petrić, Ines; Lončar, Jovica; Smital, Tvrtko; Ahel, Marijan; Terzić, Senka

doi:10.1016/j.scitotenv.2019.06.118

Pregled bibliografske jedinice broj: 1007411

Aerobic biodegradation of tramadol by pre- adapted activated sludge culture: Cometabolic transformations and bacterial community changes during enrichment

Kostanjevečki, Petra; Petrić, Ines; Lončar, Jovica; Smital, Tvrtko; Ahel, Marijan; Terzić, Senka

Aerobic biodegradation of tramadol by pre- adapted activated sludge culture: Cometabolic transformations and bacterial community changes during enrichment // Science of the total environment, 687 (2019), 858-866 doi:10.1016/j.scitotenv.2019.06.118 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1007411 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Aerobic biodegradation of tramadol by pre- adapted activated sludge culture: Cometabolic transformations and bacterial community changes during enrichment

Autori
Kostanjevečki, Petra ; Petrić, Ines ; Lončar, Jovica ; Smital, Tvrtko ; Ahel, Marijan ; Terzić, Senka

Izvornik
Science of the total environment (0048-9697) 687 (2019); 858-866

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Tramadol ; Biotransformation ; Taxonomic characterization ; Sludge culture ; Transformation products ; Ecotoxicological assessment

Sažetak
The biodegradation of biorecalcitrant opioid drug tramadol (TRAM) was studied in a model biodegradation experiment performed with an enriched activated sludge culture pre-adapted to high concentration of TRAM (20mg/L). TRAMand its transformation products (TPs)were determined by applying ultrahigh- performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF-MS), the sludge culture was characterized using a 16S rRNA gene amplicon sequencing, whereas ecotoxicological evaluation was performed based on determination of toxicity to freshwater algae. Tramadol removal was much faster (t1/2=1.3 days) and more efficient in glucose-containing mineral medium (cometabolic conditions) than in a medium without glucose. The elimination of the parent compound resulted in the formation of five TPs, two of which (TP 249 and TP 235) were identified as N- desmethyltramadol (N-DM TRAM) and N, N- didesmethyltramadol (N, N- diDM TRAM). The remaining 3 TPs (TP 277a-c) were isomeric compounds with an elemental composition of protonated molecules C16H24NO3 and a putative structure which involved oxidativemodification of the dimethylamino group. Pronounced changes in the taxonomic composition of the activated sludge were observed during the enrichment, especially regarding an enhanced percentage of 8 genera (Bacillus, Mycobacterium, Enterobacter, Methylobacillus, Pedobacter, Xanthobacter, Leadbetterella and Kaistia), which might be related to the observed transformations. The removal of TRAMresulted in proportional reduction of algal toxicity, implying a positive result of the accomplished transformation processes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Interdisciplinarne prirodne znanosti

POVEZANOST RADA

Projekti:
HRZZ-IP-2014-09-7031 - Sveoubuhvatna procjena ponašanja i sudbine farmaceutski aktivnih tvari u okolišu: makrolidni antibiotici i opioidni analgetici (COMPASS) (Terzić, Senka, HRZZ - 2014-09) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Petra Kostanjevečki (autor)