Naslov
Determination of killer activity of different wine yeasts

Autori
Runjić-Perić, Vjera ; Radovančić, Ivana ; Marić, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
18th ISSY, Yeast Nutrition and Natural Habitats. Book of Abstracts / Raspor, Peter et al. - Ljubljana : Biotechnical Faculty, Ljubljana, 1997, P8 -18

Skup
18th International Specialized Symposium on Yeast, Yeast Nutrition and Natural Habitats.

Mjesto i datum
Bled, Slovenija, 24.08.1997. - 29.08.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
yeasts; wine; killer activity

Sažetak
The suitability of killer yeast strains in wine making has been the centre of attention in countries where selected yeast cultures are used for induction of must fermentation. This atention has intensified since the observation that yeasts which are naturally present in the must play significant roles in supposedly pure culture fermentations. These natural yeasts include species from the genera Kloeckera, Candida, Hansenula and Saccharomyces. In this work five Saccharomyces strains, two Candida strains, as well as Kloeckera apiculata A-68 and Hansenula anomala Z-195 (some isolated from grape and musts, and all deposited in Culture Collection of Faculty of Food Technology and Biotechnology, Zagreb) were tested for killer activity. A known killer strain Sacharomyces cerevisiae K2-399, and sensitive strain Sacharomyces cerevisiae S-467 were used as reference in determination of killer activity by agar plate assay (killer activity is defined by a clear zone diameter of sensitive yeast growth inhibition). Among tested yeasts two strains, i. e. Sacharomyces cerevisiae Z-2 and Sacharomyces cerevisiae Z-5 were determined as killer yeasts with 96,5% and 92,4% of killer activity with reference to K2-399 strain. Four strains were sensitive on killer yeast toxin and three strains were neutral. The higher killer yeast of referent strain was determined during anaerobic growth in malt extract, at pH 4,5. The maximal values of clear zones were achieved at the end of the exponential growth phase, whereas the maximal killer activities of the other two killer strains were obtained at the beginning (strain Z-2) or in the middle (strain Z-5) of the exponential growth phase.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA