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Pregled bibliografske jedinice broj: 1006917

Model-based optimization of the enzymatic aldol addition of propanal to formaldehyde: a first step towards enzymatic synthesis of 3-hydroxybutyric acid

Česnik, Morana; Sudar, Martina; Roldan, Raquel; Hernandez, Karel; Parella, Teodor; Clapés, Pere; Charnock, Simon; Vasić-Rački, Đurđa; Findrik Blažević, Zvjezdana
Model-based optimization of the enzymatic aldol addition of propanal to formaldehyde: a first step towards enzymatic synthesis of 3-hydroxybutyric acid // Chemical engineering research & design, 150 (2019), 140-152 doi:10.1016/j.cherd.2019.06.025 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1006917 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Model-based optimization of the enzymatic aldol addition of propanal to formaldehyde: a first step towards enzymatic synthesis of 3-hydroxybutyric acid

Autori
Česnik, Morana ; Sudar, Martina ; Roldan, Raquel ; Hernandez, Karel ; Parella, Teodor ; Clapés, Pere ; Charnock, Simon ; Vasić-Rački, Đurđa ; Findrik Blažević, Zvjezdana

Izvornik
Chemical engineering research & design (0263-8762) 150 (2019); 140-152

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aldol addition ; D-fructose-6-phosphate aldolase ; mathematical model ; optimization ; methacrylic acid

Sažetak
3-Hydroxyisobutyric acid is an important intermediate in the biosynthesis of methacrylic acid. Its biocatalytic synthesis can be performed by aldolase-catalysed aldol addition of propanal to formaldehyde followed by an enzymatic oxidation of the resulting 3-hydroxy-2-methylpropanal to 3- hydroxyisobutyric acid. In this work, D-fructose- 6-phosphate aldolase D6Q variant was investigated as a key step for the biocatalytic preparation of 3-hydroxy-2-methylpropanal, a commercially unavailable precursor of 3-hydroxyisobutyric acid. The kinetic model of this step was developed for the purpose of reactor selection and process optimization. It was found that enzyme operational stability decay is co-dependent on the initial formaldehyde concentration. Thus, the choice of the initial conditions is crucial for a successful process set-up. It was concluded that fed-batch was the best reactor choice for this reaction due to enzyme inhibition by formaldehyde and propanal, and its operational stability decay. At the optimal process conditions, the product concentration, product yield, and volume productivity after 5.5 hours were 72 g L–1, 88.5% and 313.7 g L–1 d–1, respectively. Enzymatic oxidation of 3-hydroxy-2-methylpropanal to the corresponding acid was performed as a proof of concept using an aldehyde dehydrogenase in the presence of NAD+, regenerated by water-forming NADH oxidase, and 2.5 g L–1 (24 mM) of 3- hydroxyisobutyric acid was obtained.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

Napomena
This work has received funding from the EU Horizon
2020 research and innovation programme under grant
agreement 635595 "CarbaZymes".



POVEZANOST RADA

Projekti:
EK-H2020-635595 - Sustainable industrial processes based on a C-C bond-forming enzyme platform (CARBAZYMES) (Findrik Blažević, Zvjezdana, EK - H2020-LEIT-BIO-2014-1) ( CroRIS)

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Profili:

Avatar Url Martina Sudar (autor)

Avatar Url MORANA ČESNIK KATULIĆ (autor)

Avatar Url Zvjezdana Findrik Blažević (autor)

Avatar Url Đurđa Vasić-Rački (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com dx.doi.org

Citiraj ovu publikaciju:

Česnik, Morana; Sudar, Martina; Roldan, Raquel; Hernandez, Karel; Parella, Teodor; Clapés, Pere; Charnock, Simon; Vasić-Rački, Đurđa; Findrik Blažević, Zvjezdana
Model-based optimization of the enzymatic aldol addition of propanal to formaldehyde: a first step towards enzymatic synthesis of 3-hydroxybutyric acid // Chemical engineering research & design, 150 (2019), 140-152 doi:10.1016/j.cherd.2019.06.025 (međunarodna recenzija, članak, znanstveni)
Česnik, M., Sudar, M., Roldan, R., Hernandez, K., Parella, T., Clapés, P., Charnock, S., Vasić-Rački, Đ. & Findrik Blažević, Z. (2019) Model-based optimization of the enzymatic aldol addition of propanal to formaldehyde: a first step towards enzymatic synthesis of 3-hydroxybutyric acid. Chemical engineering research & design, 150, 140-152 doi:10.1016/j.cherd.2019.06.025.
@article{article, author = {\v{C}esnik, Morana and Sudar, Martina and Roldan, Raquel and Hernandez, Karel and Parella, Teodor and Clap\'{e}s, Pere and Charnock, Simon and Vasi\'{c}-Ra\v{c}ki, \DJur\dja and Findrik Bla\v{z}evi\'{c}, Zvjezdana}, year = {2019}, pages = {140-152}, DOI = {10.1016/j.cherd.2019.06.025}, keywords = {aldol addition, D-fructose-6-phosphate aldolase, mathematical model, optimization, methacrylic acid}, journal = {Chemical engineering research and design}, doi = {10.1016/j.cherd.2019.06.025}, volume = {150}, issn = {0263-8762}, title = {Model-based optimization of the enzymatic aldol addition of propanal to formaldehyde: a first step towards enzymatic synthesis of 3-hydroxybutyric acid}, keyword = {aldol addition, D-fructose-6-phosphate aldolase, mathematical model, optimization, methacrylic acid} }
@article{article, author = {\v{C}esnik, Morana and Sudar, Martina and Roldan, Raquel and Hernandez, Karel and Parella, Teodor and Clap\'{e}s, Pere and Charnock, Simon and Vasi\'{c}-Ra\v{c}ki, \DJur\dja and Findrik Bla\v{z}evi\'{c}, Zvjezdana}, year = {2019}, pages = {140-152}, DOI = {10.1016/j.cherd.2019.06.025}, keywords = {aldol addition, D-fructose-6-phosphate aldolase, mathematical model, optimization, methacrylic acid}, journal = {Chemical engineering research and design}, doi = {10.1016/j.cherd.2019.06.025}, volume = {150}, issn = {0263-8762}, title = {Model-based optimization of the enzymatic aldol addition of propanal to formaldehyde: a first step towards enzymatic synthesis of 3-hydroxybutyric acid}, keyword = {aldol addition, D-fructose-6-phosphate aldolase, mathematical model, optimization, methacrylic acid} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

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  • CA Search (Chemical Abstracts)

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