Naslov
Functional and protein expression of multixenobiotic resistance mechanism in a marine mussel as a biomarker of exposure to polluted environments

Autori
Britvić, Smiljana ; Krča, Sanja ; Kurelec, Branko ; Lucić, Davor ; Pivčević, Branka ; Smital, Tvrtko

Izvornik
MAP Technical Report Series No. 103, UNEP, Athens, pp. 29-41

Vrsta, podvrsta
Ostale vrste radova, ostali članci/prilozi

Godina
1998

Ključne riječi
Mytilus galloprovincialis ; multixenobiotic resistance ; P-glycoprotein ; functional analysis ; immunodetection ; induction ; biomarker

Sažetak
The expression of the multixenobiotic resistance mechanism (MXRM) in the marine mussel Mytilus galloprovincialis living along the pollution gradient in the Rovinj sea area was measured. The MXRM was induced in mussels at polluted sites: gills from mussels living at polluted sites accumulated less vincristine ; the vincristine accumulation was less sensitive to verapamil, and in most cases the expressed higher levels of P-glycoprotein. The state of induction of functional activity of MXRM is proportional to the level of pollution, as demonstrated at sites along the well defined pollution gradient in the Rovinj sea area, Northern Adriatic. Since the immunological expression of P-glycoprotein may be compromised by high interindividual defferences, season, and some as yet unknown factors, it could not be used as a single marker of exposure. However, the rate of vincristine accumulation and its sensitivity to verapamil offer a new, informatively powerful, molecular biomarker of exposure.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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