Naslov
Quantitative transplantation assays of the rat rhabdomyosarcoma BA1112 isografts into the WAG/Rij Y rat and xenotransplantation into the athymic NCr(nu/nu) nude mouse

Autori
Suit, Herman D. ; Rockwell, Sara ; Zeitman, Anthony ; Silobrčić, Vlatko ; Porter, Elizabeth A. ; Ramsay, Jonathan ; Sedlaček, Robert

Izvornik
European journal of cancer & clinical oncology (0277-5379) 25 (1989), 10; 1463-1466

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
animal cellanimal experiment ; isotransplantation ; mouse ; nonhuman ; ratrhabdomyosarcoma ; tumor cell line ; tumor xenograft

Sažetak
Quantitative tumor cell transplantation assays have been performed to compare the transplantability of rat rhabdomyosarcoma BA1112 into isologous WAG/Rij Y rats and athymic NCr(nu/nu) nude mice. The end-point was the td50 or the number of viable tumor cells which would transplant the tumors into half of the recipients. At Yale, two sets of 2-fold dilutions were prepared, one was sent to the MGH by Air Express. That afternoon, concurrent assays were performed at Yale using the WAG/Rij Y rat and at MGH using the NCr(nu/nu) mouse. The td50 values were the same for iso- and xenotransplantation. Furthermore, the td50s in rats and mice were unaffected by standard immunization procedures prior to challenge of the td50 assay. The BA1112 (107 trypan blue excluding cells) grew to 10–12 mm and then completely regressed if transplanted into NCr(nu/+) mice which had received 6 Gy whole body irradiation but did not grow in control NCr(nu/+) mice. The times for the BA1112 to grow to 10 mm were the same in normal or preimmunized WAG/Rij Y rats or NCr(nu/nu) mice and in 6 Gy WBI NCr(nu/nu) mice. All of the experimental data show that the xenogenic NCr(nu/nu) mice accept the BA1112 as readily as do the isologous WAG/Rij Y rats.

Izvorni jezik
Engleski

Znanstvena područja
Informacijske i komunikacijske znanosti

Napomena
Rad je djelomično prezeniran na skupu Proceedings of the American Association of Cancer Research (1987) (28) str. 337 ; Abs. No. 1334.

POVEZANOST RADA