Naslov
Characterization of organic anion transporters in zebrafish (Danio rerio Hamilton, 1822)

Autori
Dragojević, Jelena

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Sveučilište u Osijeku

Mjesto
Osijek

Datum
14.12

Godina
2018

Stranica
110

Mentor
Smital, Tvrtko

Ključne riječi
SLC transporters ; Danio rerio ; phylogenetic analysis ; synteny analysis ; tissue expression ; function ; ADME ; CRISPR/Cas9

Sažetak
Organic anion transporters (OATPs/OATs) are transmembrane proteins which belong to SLC21 and SLC22 subfamily of the SLC (Solute Carriers) family. They are responsible for the uptake of various endogenous and xenobiotic compounds into the cell. Despite their crucial role in toxicological response, their detailed ecotoxicological relevance and characteristics in non-mammalians are still unknown. Therefore, the main goal of this study was identification and molecular characterization of organic anion transporters in zebrafish (Danio rerio). Additional goal was the creation of zebrafish gene knockouts for the best characterized zebrafish transporter of organic anions, Oatp1d1. Seven organic anion transporters are present in zebrafish: Oat1, Oat2a, 2b, 2c, 2d, 2e and Oat3, encoded by genes slc22a6, slc22a7(a-e) and slc22a8, respectively. New model fluorescent substrates were identified for 5 zebrafish Oats, and high throughput in vitro assays for identification of interactors of these proteins were developed. Zebrafish Oats showed interaction with various endo- and xenobiotics. The first CRISPR/Cas9 zebrafish Oatp1d1 knockouts were succesfuly generated.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA