Naslov
The Interaction of Fullerenol C60(OH)24Nanoparticles with the Aflatoxigenic Foodborne Fungi

Autori
Kovač, Tihomir ; Borišev, Ivana ; Lončarić, Ante ; Ezekiel, Chibungu Ngozi ; Sulyok, Michael ; Krska, Rudolf ; Šarkanj, Bojan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Young Scientists Days –Young Scientists Conference Book of abstracts / Stanković, Anamarija ; Šafranko, Silvija ; Štolfa Čamagajevac, Ivna ; Žuna Pfeiffer, Tanja ; Živić, Tihomir - Osijek : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2019, 30-30

Skup
2. dani mladih istraživača = 2nd Young Scientists Days Conference

Mjesto i datum
Osijek, Hrvatska, 23.05.2019. - 24.05.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Recenziran

Ključne riječi
fullerenol C60(OH)24nanoparticles, Aspergillus flavus, aflatoxin biosynthetic pathway, emerging toxins, global climaticchange

Sažetak
The fullerenols, C60(OH)24(FNPs), are the water‐soluble fullerene C60daughter products that possessa great potential for amodulationof biosynthetic pathways of the secondary metabolites in the foodborne aflatoxigenic fungi. In order to clarify the extent, anFNP–aflatoxigenicfungi interaction, the impact of different FNP concentrations (0, 10, 100 and 1, 000 ng mL-1) on an aflatoxin biosynthesis pathway, and a production of emerging toxins (a non‐aflatoxin biosynthetic pathway) from Aspergillus flavusNRRL 3251 were examined. The fungi werecultivatedat 29 °C in thedark, in an extract‐sucrose (YES) medium during a 168‐hourgrowth period. Adiameter of the FNPs used in this study amounted to8 nm, while adetermined ζ potential amounted to-33 mV. A mycelial growth was affected at 1, 000 ng mL-1, while a conidialproduction was slightly affected at 10 ng mL-1. The mycelia and media samplescontained most of the monitored aflatoxin precursors, with an exception of the norsorolinic acid. Furthermore, the observed FNP effect was concentration‐dependent, and a sterigmatocystin export from the cells was altered. An antioxidative effect of FNPs subsequent to120 hoursof the growth period vanished and caused a strong concentration‐dependent aflatoxigenic effect tillthe end of the growth period (168 hrs). In case of the emerging toxins, ten of them were produced in a sum of total concentrations reaching 1, 745, 035 ng 50 mL-1of the YES medium, while three of them were found only in the mycelia. The observed ratio of mycelia:media distribution amounted to99:1. An amount of1, 000 ng mL-1of FNPs increased the medial concentrations, while an increase inthe applied concentrations of FNPs provokeda decrease of the observed mycelial‐emerging toxin concentrations. In addition, cordycepin was confirmed as an A. flavussecondary metabolite for the first time. Anoutcome of the presented study pointsout the relevance of a risk assessment concerning thenanoparticles and their availability in the environment in the time of global climaticchanges.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

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