Naslov
Anxiolytic effect BPC-157 a gastric pentadecapeptide: shock probe/burying and light/dark test.

Autori
Sikirić, Predrag ; Jelovac, Nikola ; Jelovac-Gjeldum, Andjelka ; Dodig, Goran ; Starešinić, Mario ; Anić, Tomislav ; Zoričić, Ivan ; Perović, Darko ; Aralica, Gorana ; Buljat, Gojko ; Prkačin, Ingrid ; Lovrić-Benčić, Martina ; Šeparović, Jadranka ; Seiwerth, Sven ; Ručman, Rudolf ; Petek, Marijan ; Turković, Branko

Izvornik
Acta pharmacologica Sinica (1671-4083) 22 (2001), 3; 225-30

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Gastric pentadecapeptide BPC 157; diazepam; shock probe/burying and light/dark test; particular anxiolytic effect

Sažetak
We reported for gastric pentadecapeptide BPC 157 GEPPPGKPADDAGLV, M.W. 1419, a anxiolytic effect in shock probe/ burying test (no burying, lack of panic reaction) and in light/dark test (no effect in light areas, and particularly increased activity in dark zone). Possible anxiolytic effect is along with proposed pentadecapeptide BPC 157-regulation of dopamine mediated events within extrapyramidal system and behaviors dependent on striatal functions (as follows from antagonization of neuroleptic catalepsy, amphetamine stereotypes, antidepressant effect, haloperidol/amphetamine climbing behavior, MPTP and reserpine motor disturbances), and possible role of pentadecapeptide BPC 157 in stress reaction. In shock probe/burying test pentadecapeptide BPC 157 (10 ľg, 10 ng/kg b.w. i.p), diazepam (0.075, 0.0375 mg/kg b.w. i.p.), an equivolume of saline (5 ml/kg b.w. i.p.) were given at 30 min prior, rats were individually placed into the test box, observation for 15 min. Rats treated with either diazepam or pentadecapeptide BPC 157 were much less afraid after the shock: almost not burying and the total time spent in burying was clearly less than in controls. However, while in the diazepam treated rats the number of shocks received increased over control values, in pentadecapeptide BPC 157 treated groups the number of shocks remained not modified compared with the control values. In light/dark test, medication (pentadecapeptide BPC 157 (10 ľg, 10 ng/kg b.w. i.p), diazepam (0.1 mg/kg b.w. i.p.), an equivolume of saline (5 ml/kg b.w. i.p.)) was given at 45 min prior procedure, each mouse was tested by placing it in the centre of the white area and allowing it to explore the novel environment for 10 minutes. After exposure to the intense light, diazepam treated mice had longer latencies of crossing to the dark compartment, a greater number of crossing and a greater number of exploratory rearing, and spent longer time in the light compartment, as compared to the control mice, while BPC 157 mice had a similar behavior to that of the control mice. In contrast with the effect in light area, in dark zone diazepam produced no change with respect to controls, while BPC 157 (10 ľg) mice had a greater number of crossing and a greater number of exploratory rearing. Thus, both agents displayed a bidirectional antianxiety effect (measured by an increase of in specific activity in one paradigm (a light/dark test), and a decrease in a specific activity in the other paradigm (shock probe/burying)), thereby not related to nonspecific effects on general activity or arousal. However, at the same time, these indicate anxiolytic activity of pentadecapeptide BPC 157 to be particular, and different from diazepam. Therefore, in relation with its other behavioral effects, the particular anxiolytic activity of pentadecapeptide BPC 157 in anxiety models is fully compatible with a general statement that drugs acting at various target areas might be expected to provide selective actions on some but not all of the somatic symptoms associated with anxiety.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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