Naslov
Advances in cyanine - Amino acid conjugates and peptides for sensing of DNA, RNA and protein structures

Autori
Šmidlehner, Tamara ; Rožman, Andrea ; Piantanida, Ivo

Izvornik
Current protein & peptide science (1389-2037) 20 (2019), 11; 1040-1045

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA/RNA recognition ; protein recognition ; fluorescence ; cyanine - amino acid conjugate

Sažetak
Small molecules - spectrophotometric probes for DNA/RNA and proteins are of the utmost importance for the diagnostics in biochemical and biomedical researches. Both, naturally occurring and synthetic probes, often include peptide sequence responsible for the selectivity toward the particular target ; however, commercially available dyes are restricted to single point attachment to the peptide (having one reactive group). Here presented are our recent advances in the development of novel amino acid-fluorophore probes, with the unique characteristic of free N- and C-terminus available for incorporation at any peptide backbone position. Intriguingly, already monomeric amino acid-fluorophores showed recognition among various DNA/RNA, whereby steric impact and contribution of halogens is systematically studied. Moreover, some dyes revealed intracellular mitochondria specificity. Further, several hetero-dimeric chromophore systems were prepared, demonstrating that synergistic effect can lead to simultaneous DNA, RNA and protein fluorimetric recognition, combined with enzyme inhibition. Also, homodimeric cyanines equipped with chlorine revealed intriguing DNA/RNA selectivity in respect to well-known parent TOTO and YOYO dyes.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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