Naslov
The role of Semaphorin5A protein during brain development in rodents

Autori
Zima, David ; Putar, Darija ; Trnski, Sara ; Bobić Rasonja, Mihaela ; Jovanov Milošević, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Rad Hrvatske akademije znanosti i umjetnosti. Medicinske znanosti, 537 (2019), 46-47 / - Zagreb, 2019, 76-76

Skup
Nikola Škreb Symposium: „New platforms in developmental biology - towards the clinical application“

Mjesto i datum
Zagreb, Hrvatska, 29.11.2018. - 30.11.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
somato-sensory cortex ; autism spectrum disorder ; Cri-du-chat ; proteoglycans

Sažetak
The Semaphorin-5A (Sema5A) is a transmembrane protein that has been shown to have a role as bifunctional axon guidance molecule during axon elongation and cell migration in the development of diencephalon and its connections. Mutations of Sema5A are linked to the etiology of some disorders and syndromes, as microdeletion of SEMA5A found in patients with autism spectrum and haploinsufficiency for SEMA5A in Cri-du-chat syndrome. We are lacking data on SEMA5A protein expression in human brain but according to the recent data (Allan Brain Institute), the transcripts of Sema5A gene are present in proliferative zones of the developing telencephalon in rodents at age E13.5 E15.5 and P14. In the present study, we used in vitro functional essay and post-mortem protein expression analysis to reveal possible role of Sema5A in mammalian brain development. The neocortical explants from mouse embryo (E15) were cultivated 72h on Sema5a uniform or alternately presenting carpet (made of membrane homogenate of Sema5A transfected Human Embryonic Kidney 293 cells.) Results of the in vitro study show that Sema5A promotes outgrowth and elongation of neocortical axons when uniformly presented on the carpet, but does not steer axons or modulate collateralization on Sema5A- alternating carpets. The expression of Sema5A protein revealed by immunohistochemical staining was more prominent at P15 than at P5 in the entire cortex with the clear regional differences in the expression. The most prominent change in expression was in somatosensory cortex suggesting a role of Sema5a in the development of the thalamocortical connections and the area in the observed early postnatal period. Further postmortem studies are needed to get a better understanding of Sema5A interaction with specific proteoglycans in the extracellular matrix, which subsequently would lead to a disclosure of the Sema5A role in the development of the telencephalon connectivity in health and disease. The study was co- financed by the European Union through the European Regional Development Fund, Operational Programme Competitiveness, and Cohesion, grant agreement No. KK.01.1.1.01.0007, CoRE and Croatian Science Foundation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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