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Pregled bibliografske jedinice broj: 1000844

EFFECTS OF CIGARETTE SMOKE EXTRACT ON INDUCTION OF TIMP-1 AND MMP-9 IN HUMAN A549 CELL LINE


Somborac, Anita; Žanić-Grubišić, Tihana
EFFECTS OF CIGARETTE SMOKE EXTRACT ON INDUCTION OF TIMP-1 AND MMP-9 IN HUMAN A549 CELL LINE // ''Hottest topics in protein research''
Split, 2008. str. 47-47 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1000844 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
EFFECTS OF CIGARETTE SMOKE EXTRACT ON INDUCTION OF TIMP-1 AND MMP-9 IN HUMAN A549 CELL LINE

Autori
Somborac, Anita ; Žanić-Grubišić, Tihana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
''Hottest topics in protein research'' / - Split, 2008, 47-47

Skup
MedILS Summer School '08

Mjesto i datum
Split, Hrvatska, 19.07.2008. - 26.07.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
A549, cigarette smoke, COPD, MMP-9, TIMP-1

Sažetak
Background: Chronic obstructive pulmonary disease (COPD) is the most frequent chronic respiratory disease with the progressive course. It is characterised with the development of local and presumably systemic inflammation. Cigarette smoking is the main etiologic factor causing the inflammatory response in COPD patients. MMP-9 can play an important role in inflammation and lung tissue remodelling owing to its gelatinolitic activity. TIMP-1 is known as an inhibitor of matrix metalloproteinases, including MMP-9. In this study the effect of cigarette smoke extract (CSE) on induction of TIMP-1 and MMP-9 in human A549 cell line was examined. Materials and Methods: Human A549 cell line (lung carcinoma epithelial cells) were cultured in F-12 medium supplemented with 10 % FBS, 1 % GlutaMAX and 1 % Antibiotic-Antimycotic. The cigarette smoke extract was freshly prepared at the day of use by slowly bubbling the cigarette smoke through the F-12 medium. Cells (2 x 106 / well) were treated with different concentrations of CSE for 4h and 24h at 37°C. Supernatant was collected and frozen at -20°C until determination. TIMP-1 and MMP-9 were determined with the ELISA kit. Results: CSE induces the release of TIMP-1 from A549 cells after 24h incubation. Basal production of MMP-9 in A549 cells is very low and there was no induction of MMP-9 with any concentration of CSE. Conclusions: Human A549 cells stimulated with CSE release high concentrations of TIMP-1. In contrast to our expectations, CSE activated A549 cells do not release MMP-9. Further examination is needed in order to get more significant results.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekti:
006-0061245-0977 - Molekularni mehanizmi patogeneze kronične opstrukcijske bolesti pluća (Žanić-Grubišić, Tihana, MZOS ) ( CroRIS)

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb


Citiraj ovu publikaciju:

Somborac, Anita; Žanić-Grubišić, Tihana
EFFECTS OF CIGARETTE SMOKE EXTRACT ON INDUCTION OF TIMP-1 AND MMP-9 IN HUMAN A549 CELL LINE // ''Hottest topics in protein research''
Split, 2008. str. 47-47 (poster, međunarodna recenzija, sažetak, znanstveni)
Somborac, A. & Žanić-Grubišić, T. (2008) EFFECTS OF CIGARETTE SMOKE EXTRACT ON INDUCTION OF TIMP-1 AND MMP-9 IN HUMAN A549 CELL LINE. U: ''Hottest topics in protein research''.
@article{article, author = {Somborac, Anita and \v{Z}ani\'{c}-Grubi\v{s}i\'{c}, Tihana}, year = {2008}, pages = {47-47}, keywords = {A549, cigarette smoke, COPD, MMP-9, TIMP-1}, title = {EFFECTS OF CIGARETTE SMOKE EXTRACT ON INDUCTION OF TIMP-1 AND MMP-9 IN HUMAN A549 CELL LINE}, keyword = {A549, cigarette smoke, COPD, MMP-9, TIMP-1}, publisherplace = {Split, Hrvatska} }
@article{article, author = {Somborac, Anita and \v{Z}ani\'{c}-Grubi\v{s}i\'{c}, Tihana}, year = {2008}, pages = {47-47}, keywords = {A549, cigarette smoke, COPD, MMP-9, TIMP-1}, title = {EFFECTS OF CIGARETTE SMOKE EXTRACT ON INDUCTION OF TIMP-1 AND MMP-9 IN HUMAN A549 CELL LINE}, keyword = {A549, cigarette smoke, COPD, MMP-9, TIMP-1}, publisherplace = {Split, Hrvatska} }




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