Naslov
Spreading of Alfalfa mosaic virus in Lavandin in Croatia

Autori
Vrandečić, Karolina ; Ćosić, Jasenka ; Stanković, Ivana ; Milojević, Katarina ; Bulajić, Aleksandra Krstić, ranka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of Joint International Congress of the Mediterranean Phytopathological Union and International Society of Mycotoxicology / - , 2014, 112-112

Skup
14th MPU Congress

Mjesto i datum
Istanbul, Turska, 25.08.2014. - 29.08.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Alfalfa mosaic virus ; lavandin ; Croatia

Sažetak
In 2012 and 2013, a survey was conducted to detect the occurrence and distribution of Alfalfa mosaic virus (AMV) in lavandin crops growing in continental parts of Croatia. A total of 73 lavandin samples from six crops in different localities were collected and analyzed for the presence of AMV and Cucumber mosaic virus (CMV) using commercial double- antibody sandwich (DAS)-ELISA kits. AMV was detected in 62 samples collected at three different localities, and none of the samples tested positive for CMV. For further analyses, six selected samples of naturally infected lavandin plants originating from different localities were successfully mechanically transmitted to test plants Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana, and Ocimum basilicum, confirming the infectious nature of the viruses. Molecular detection was performed by amplification of a fragment of 751 bp in all tested samples, using the specific primers CP AMV1/CP AMV2 that amplify the part of coat protein (CP) gene. The RT-PCR products derived from isolates 371-13 and 373-13 were sequenced (KJ504107 and KJ504108, respectively) and compared with the AMV sequences available in GenBank. CP sequence analysis, conducted using MEGA5 software, revealed that isolate 371-13 showed the highest nucleotide of 99.5% (100% amino acid identity) with an isolate from Argentina originating from Medicago sativa (KC881010), and the sequence of isolate 373-13 had the highest identity with Italian AMV isolate from Lavandula stoechas (FN667967) of 98.6% (99% amino acid identity). Phylogenetic analysis showed clustering of selected isolates into four molecular groups and lavandin AMV isolates from Croatia grouped into two distinct groups. Isolates 70-12 and 373-13 clustered in group IV, together with the majority of isolates selected for phylogenetic analysis, while isolate 371-13 grouped in molecular group III. Determination of variability within the population of AMV in lavandin crops, but also establishing relationships with the isolates originating from other host plants in Croatia, will contribute to better understanding of the epidemiology of this pathogen, especially related to virus reservoirs in nature and the way the virus is introduced into crops.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA