Pregled bibliografske jedinice broj: 999851
Metabolomics reveals citric acid secretion in mechanically–stimulated osteocytes is inhibited by high glucose
Metabolomics reveals citric acid secretion in mechanically–stimulated osteocytes is inhibited by high glucose // Scientific Reports, 9 (2019), 1; 2295-2305 doi:10.1038/s41598-018-38154-6 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 999851 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Metabolomics reveals citric acid secretion in mechanically–stimulated osteocytes is inhibited by high glucose
Autori
Villaseñor, Alma ; Aedo-Martín, Daniel ; Obeso, David ; Erjavec, Igor ; Rodríguez-Coira, Juan ; Buendía, Irene ; Ardura, Juan Antonio ; Barbas, Coral ; Gortazar, Arancha R.
Izvornik
Scientific Reports (2050-1218) 9
(2019), 1;
2295-2305
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Metabolomics, osteocytes, citric acid, mechanical stimulation
Sažetak
Osteocytes are the main cells of bone tissue and play a crucial role in bone formation and resorption. Recent studies have indicated that Diabetes Mellitus (DM) affects bone mass and potentially causes higher bone fracture risk. Previous work on osteocyte cell cultures has demonstrated that mechanotransduction is impaired after culture under diabetic pre-conditioning with high glucose (HG), specifically osteoclast recruitment and differentiation. The aim of this study was to analyze the extracellular metabolic changes of osteocytes regarding two conditions: pre-conditioning to either basal levels of glucose (B), mannitol (M) or HG cell media, and mechanical stimulation by fluid flow (FF) in contrast to static condition (SC). Secretomes were analyzed using Liquid Chromatography and Capillary Electrophoresis both coupled to Mass Spectrometry (LC-MS and CE-MS, respectively). Results showed the osteocyte profile was very similar under SC, regardless of their pre-conditioning treatment, while, after FF stimulation, secretomes followed different metabolic signatures depending on the pre-conditioning treatment. An important increment of citrate pointed out that osteocytes release citrate outside of the cell to induce osteoblast activation, while HG environment impaired FF effect. This study demonstrates for the first time that osteocytes increase citrate excretion under mechanical stimulation, and that HG environment impaired this effect.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Interdisciplinarne biotehničke znanosti
